Viral Manipulation of Neural Stem/Precursor Cells
Viral Manipulation of Neural Stem/Precursor Cells
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DOI：
10.1007/978-1-62703-610-8_14
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Pluchino, Stefano
中科院分区：
文献类型：
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作者：
Iraci, Nunzio;Tyzack, Giulia Elisabetta;Pluchino, Stefano
神经干细胞/前体细胞(NPC)是根据其自我更新和分化为中枢神经系统(CNS)所有主要细胞类型的能力来定义的。它们可以在特定的培养条件下以球形聚集体(神经球)的形式存活和增殖，传代次数几乎不确定，同时保持表型和核型的稳定。除了经典的以干细胞为基础的再生医学方法外，神经干细胞在移植后作为体内分泌因子和/或治疗基因的传递系统也具有巨大的潜力。确凿的证据表明，神经干细胞在宿主中枢神经系统内的移植过程中存活下来，特别是在受损组织中迁移，并保护神经系统免受炎症和其他形式的损害。病毒载体是操纵和改善鼻咽癌某些特性的通用工具，包括它们的迁移和分化能力，从而允许许多不同的可能的应用。在这一章中，我们描述了一些制备鼻咽癌细胞的方法，慢病毒颗粒的产生和滴定，以及病毒介导的鼻咽癌细胞的体外培养和体内操作。
Neural stem/precursor cells (NPCs) are defined according to their ability to self-renew and differentiate into all the major cellular types of the central nervous system (CNS). They can survive and proliferate in defined culture conditions as spherical aggregates (neurospheres) for a virtually indefinite number of passages, while remaining phenotypically and karyotypically stable. Besides classical stem cell-based approaches for regenerative medicine, NPCs hold a great potential also as system for delivering secreted factors and/or therapeutic genes in vivo, after transplantation. Solid evidence exist that NPCs survive transplantation procedures within the host CNS, specifically migrate within the damaged tissue, and protect the nervous system from inflammatory and other forms of damage. Viral vectors constitute a versatile tool for manipulating and improving some of the NPC properties, including their migratory and differentiation capacities, thus allowing a number of different possible applications. In this chapter, we have described some of the methods for the preparation of NPCs, the production and titration of the lentiviral particles, and the viral-mediated manipulation of NPCs, both ex vivo in organotypic slice cultures, as well as in vivo.