Molecular organization and embryonic expression of the hedgehog gene involved in cell-cell communication in segmental patterning of Drosophila.
Molecular organization and embryonic expression of the hedgehog gene involved in cell-cell communication in segmental patterning of Drosophila.
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作者：
J. Mohler;K. Vani
hedgehog基因是维持果蝇胚胎各节正常组织所必需的节极性基因。我们已经确定了一些与刺猬突变相关的重排断点的物理位置。相应的hh RNA以一系列节段条纹的形式表达，起始于每个节段后部的细胞胚盘。这种RNA主要定位在细胞核内，直到第10阶段，此时定位主要变为细胞质。在后室的hh RNA的表达是独立的大多数其他节极性基因，包括en，直到晚期延长生殖带阶段（第11阶段）。hedgehog基因座的序列分析表明，该蛋白产物是一种跨膜蛋白，因此可能直接参与细胞间的通讯。
hedgehog is a segment polarity gene necessary to maintain the proper organization of each segment of the Drosophila embryo. We have identified the physical location of a number of rearrangement breakpoints associated with hedgehog mutations. The corresponding hh RNA is expressed in a series of segmental stripes starting at cellular blastoderm in the posterior portion of each segment. This RNA is localized predominantly within nuclei until stage 10, when the localization becomes primarily cytoplasmic. Expression of hh RNA in the posterior compartment is independent of most other segment polarity genes, including en, until the late extended germ-band stage (stage 11). Sequence analysis of the hedgehog locus suggests the protein product is a transmembrane protein, which may, therefore, be directly involved in cell-cell communication.