Activities of enzymes of carbohydrate and energy metabolism of the spores of the microsporidian, Nosema grylli
Activities of enzymes of carbohydrate and energy metabolism of the spores of the microsporidian, Nosema grylli
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DOI：
10.1111/j.1550-7408.1997.tb05707.x
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发表时间：
1997-05-01
影响因子：
2.2
通讯作者：
Issi, IV
中科院分区：
文献类型：
--
作者：
Dolgikh, VV;Sokolova, JJ;Issi, IV
利用从蟋蟀脂肪体中分离纯化的微孢子虫Nosema grylli孢子，研究了14种酶的存在。葡萄糖6-磷酸脱氢酶（EC 1.1.1.49），磷酸葡萄糖变位酶（EC 5.4.2.2），磷酸葡萄糖异构酶（EC 5.3.1.9），果糖6-磷酸激酶（EC 4.1.2.13），醛缩酶（ EC 2.7.1.11），3-磷酸甘油酸激酶（EC 2.7.2.3），丙酮酸激酶（EC 2.7.1.40）和甘油3-磷酸脱氢酶（EC 1.1.1.8）检测到的活性为15 +/- 1，7 +/-：1、1，549 +/- 255、10 +/- 1、5 +/- 1、16 +/- 4、6 +/- 1和16 +/- 2 nmol/min.mg蛋白。未检出己糖激酶（EC 2.7.1.1）、NAD依赖性苹果酸脱氢酶（EC 1.1.1.37）、苹果酸酶（EC 1.1.1.40）、乳酸脱氢酶（EC 1.1.1.27）、醇脱氢酶（EC 1.1.1.1）和琥珀酸脱氢酶（EC 1.3.99.1）。这些结果表明，微孢子虫中碳水化合物的催化作用通过Embden-Meyerhof途径发生。甘油3-磷酸脱氢酶可以再氧化由甘油醛3-磷酸脱氢酶在糖酵解中产生的NADH。
The presence of 14 enzymes was investigated using purified spores of the microsporidian Nosema grylli from fat body of the crickets Gryllus bimaculatus. Glucose 6-phosphate dehydrogenase (EC 1.1.1.49), phosphoglucomutase (EC 5.4.2.2), phosphoglucose isomerase (EC 5.3.1.9), fructose 6-phosphate kinase (EC 2.7.1.11), aldolase (EC 4.1.2.13), 3-phosophoglycerate kinase (EC 2.7.2.3), pyruvate kinase (EC 2.7.1.40) and glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) were detected with activities of 15 +/- 1, 7 +/-: 1, 1,549 +/- 255, 10 +/- 1, 5 +/- 1, 16 +/- 4, 6 +/- 1 and 16 +/- 2 nmol/min.mg protein, respectively. Hexokinase (EC 2.7.1.1), NAD-dependent malate dehydrogenase (EC 1.1.1.37), malic enzyme (EC 1.1.1.40), lactate dehydrogenase (EC 1.1.1.27), alcohol dehydrogenase (EC 1.1.1.1) and succinate dehydrogenase (EC 1.3.99.1) were not detectable. These results suggest the catabolism of carbohydrates in microsporidia occurs via the Embden-Meyerhof pathway. Glycerol 3-phosphate dehydrogenase may reoxidize NADH which is produced by glyceraldehyde 3-phosphate dehydrogenase in glycolysis.