SIMPLIFIED ANALYSIS OF BENZO(ALPHA)PYRENE IN AIRBORNE-PARTICULATES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SIMPLIFIED ANALYSIS OF BENZO(ALPHA)PYRENE IN AIRBORNE-PARTICULATES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(01)87923-1
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
YOSHIKAWA, M
中科院分区：
文献类型：
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作者：
KODAMA, Y;ARASHIDANI, K;YOSHIKAWA, M
建立了一种测定大气颗粒物中苯并[a]芘(BaP)的简便高效液相色谱分析方法。该方法包括碳氢化合物的超声提取，提取的多环芳烃(PAH)用碱性氧化铝净化，然后用带有荧光检测器的高效液相色谱分离和测定每种PAH。BaP的回收率为99%，检出限(信噪比=2)为16pg。并与日本的另一种方法进行了比较，该方法包括超声提取多环芳烃、一维双波段薄层色谱分离多环芳烃和荧光分光光度测定。两种方法对20个大气颗粒物样品的测定结果一致(r=0.998)。
A simple high-performance liquid chromatographic (HPLC) method for the determination of benzo[a]pyrene (BaP [a carcinogenic and mutagenic air contaminant] in airborne particulates is described. The method involves ultrasonic extraction of hydrocarbons, clean-up of extracted polynuclear aromatic hydrocarbons (PAH) by basic alumina, and separation and determination of each PAH by HPLC with a fluorescence detector. The recovery of BaP was 99% and the detection limit (signal-to-noise ratio = 2) was 16 pg. The results were compared with those obtained by another method used for a nationwide survey in Japan, which consists of ultrasonic extraction of PAH, separation of the PAH by 1-dimensional dual-band TLC and spectrofluorimetric determination. The results obtained by the 2 methods were in good agreement (r = 0.998) for 20 airborne particulate samples.