Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay: the effect of circadian time, age and genetic background
Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay: the effect of circadian time, age and genetic background
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DOI：
10.1016/s0196-9781(02)00251-6
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发表时间：
2002-12-01
期刊：
影响因子：
3
通讯作者：
Mignot, E
中科院分区：
文献类型：
--
作者：
Lin, L;Wisor, J;Mignot, E
下丘脑分泌素(1和2)已经成为睡眠和清醒的关键调节因子。我们开发了一种高通量酶免疫测定法(EIA)来测量大量小鼠的脑下丘脑泌素水平。下丘脑肌素水平不受昼夜节律或年龄的影响。然而，在不同品系的小鼠中，观察到一种或两种下克汀肽的显著差异。我们研究了具有肥胖、昼夜节律基因突变或单胺能缺陷的基因敲除和转基因小鼠模型中的下丘脑肌素水平。与对照组相比，只有组胺受体基因敲除的患者具有较低的下丘脑泌素水平。这在H1受体敲除中最为明显，这表明在下丘脑和组胺能神经元之间存在正反馈回路。(C)2002 Elsevier Science Inc.保留所有权利。
The hypocretins (1 and 2) have emerged as key regulators of sleep and wakefulness. We developed a high-throughput enzyme immunoassay (EIA) to measure total brain hypocretin levels from large numbers of mice. Hypocretin levels were not altered by circadian time or age. However, significant differences in one or both hypocretin peptides were observed between different mouse strains. We studied hypocretin levels in knockout and transgenic mouse models with obesity, circadian gene mutations or monoaminergic defects. Compared to controls, only histamine receptor knockouts had lower hypocretin levels. This was most pronounced in H1 receptor knockouts suggesting the existence of a positive feedback loop between hypocretin and histaminergic neurons. (C) 2002 Elsevier Science Inc. All rights reserved.