Differential expression and localization of de-novo synthesized endometriotic haptoglobin in endometrium and endometriotic lesions
Differential expression and localization of de-novo synthesized endometriotic haptoglobin in endometrium and endometriotic lesions
复制标题
DOI：
10.1093/humrep/15.10.2180
复制
发表时间：
2000-10-01
影响因子：
6.1
通讯作者：
Horowitz, GM
中科院分区：
文献类型：
--
作者：
Sharpe-Timms, KL;Ricke, EA;Horowitz, GM
应用原位杂交和免疫组化技术，对子宫内膜异位症患者的子宫内膜异位症病变和异位子宫内膜以及未患子宫内膜异位症的女性（对照组）的子宫内膜异位症蛋白- i (endoi) mRNA的表达和蛋白定位进行了研究。在分泌期，疾病女性子宫内膜异位症病变和异位子宫内膜中ENDO-I mRNA的表达显著高于疾病女性增殖期异位子宫内膜或对照组异位子宫内膜中ENDO-I mRNA的表达（P < 0.001），与周期分期无关。与对照组的异位子宫内膜相比，患者子宫内膜异位症病变和异位子宫内膜中的endoi蛋白定位更多，无论周期如何（P < 0.001）， endoi的差异表达和定位可能有助于制定子宫内膜异位症的微创诊断策略。此外，由于ENDO-I与肝珠蛋白（在某些疾病状态下具有免疫抑制和血管生成作用）具有相同的核苷酸序列和氨基酸序列，ENDO-I在腹腔中的表达和定位差异可能有助于子宫内膜异位症的发病机制和/或促进前所未有的诊断或治疗方法的发展，以管理这种神秘疾病。
Endometriosis protein-I (ENDO-I) mRNA expression and protein localization were evaluated using in-situ hybridization and immunohistochemistry in endometriotic lesions and eutopic endometrium from women with endometriosis, and in eutopic endometrium from women without endometriosis (controls), When present, ENDO-I mRNA and protein were observed in the functionalis zone of endometrial stroma and the stroma of endometriotic lesions. Expression and localization differences were scored and statistically analysed, During the secretory stage, ENDO-I mRNA expression by endometriotic lesions and eutopic endometrium from women with disease was significantly greater than ENDO-I mRNA expression by proliferative stage eutopic endometrium from women with disease or eutopic endometrium from controls, regardless of cycle stage (P < 0.001). More ENDO-I protein was localized in endometriotic lesions and entopic endometrium from women with disease than in eutopic endometrium from controls, regardless of cycle stage (P < 0.001), Differential expression and localization of ENDO-I may help develop minimally invasive diagnostic strategies for endometriosis. Further, as ENDO-I shares nucleotide sequence and amino acid sequence with hepatic haptoglobin-which in certain disease states is immunosuppressive and angiogenic-differences in ENDO-I expression and localization in the peritoneal cavity may contribute to the pathogenesis of endometriosis and/or facilitate development of unprecedented diagnostic or therapeutic approaches for management of this enigmatic disease.