Endosperm Protein Synthesis in Maize Mutants with Increased Lysine Content
Endosperm Protein Synthesis in Maize Mutants with Increased Lysine Content
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DOI：
10.1126/science.176.4042.1425
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发表时间：
1972-06
期刊：
影响因子：
56.9
通讯作者：
P. Misra;R. Jambunathan;E. Mertz;D. V. Glover;H. M. Barbosa;Kenneth S. McWhirter
中科院分区：
文献类型：
--
作者：
P. Misra;R. Jambunathan;E. Mertz;D. V. Glover;H. M. Barbosa;Kenneth S. McWhirter
玉米突变体opaque-2、opaque-7、floury-2、brittle-2以及opaque-2和brittle-2双突变体的胚乳蛋白质用Landry-Moureaux法分离为5个可溶性组分。与同基因正常胚乳相比，突变胚乳中白蛋白、球蛋白和谷蛋白-3的浓度较高，醇溶谷蛋白的浓度较低。opaque-2和brittle-2基因的组合增强了这种差异。虽然这四个突变基因位于三条不同的染色体上，但它们对胚乳蛋白质组成的影响相似。其他五个淀粉修饰突变体与高赖氨酸含量类似的脆性-2突变体的胚乳蛋白质的组成，当该基因是单独存在或结合不透明-2。因此，在具有高赖氨酸浓度的所有玉米突变体中的蛋白质合成模式可以是相同的或非常相似的。因为当面粉-2与opaque-2组合时没有获得对赖氨酸浓度的协同效应，所以导致玉米醇溶蛋白合成减少的不同途径可能存在于具有高赖氨酸浓度的面粉和淀粉修饰突变体中。
The endosperm proteins of the maize mutants, opaque-2, opaque-7, floury-2, brittle-2, and the double mutant of opaque-2 and brittle-2, were separated into five soluble fractions by the Landry-Moureaux method. As compared to their isogenic normal counterparts, the mutant endosperms had higher concentrations of albumins, globulins, and glutelin-3, and lower concentrations of prolamines. The combination of the opaque-2 and brittle-2 genes enhanced this difference. Although the four mutant genes are located on three different chromosomes, they exert a similar effect on endosperm protein composition. Five other starchmodifying mutants with high lysine content resemble the brittle-2 mutant in endosperm protein composition, when the gene is present either singly or combined with opaque-2. Therefore, the pattern of protein synthesis in all maize mutants with high lysine concentrations may be either identical or very similar. Because no synergistic effect on lysine concentration is obtained when floury-2 is combined with opaque-2, different pathways leading to reduced zein synthesis may exist in the floury and starch-modifying mutants with high lysine concentrations.