Detection of live and antibiotic-killed bacteria by quantitative real-time PCR of specific fragments of rRNA
Detection of live and antibiotic-killed bacteria by quantitative real-time PCR of specific fragments of rRNA
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DOI：
10.1128/aac.00869-05
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发表时间：
2006-06-01
影响因子：
4.9
通讯作者：
Moreillon, Philippe
中科院分区：
文献类型：
--
作者：
Aellen, Steve;Que, Yok-Ai;Moreillon, Philippe
通过分子标记物评估细菌活力可能有助于加速对抗生素诱导的杀灭的测量。本研究探讨了rRNA是否适用于此目的。将青霉素敏感性和青霉素耐受性（Tol 1突变体）戈登链球菌培养物暴露于机制不同的青霉素和左氧氟沙星。通过活菌计数评估细菌存活率，并与逆转录后16 S rRNA基因或16 S rRNA的定量实时PCR扩增进行比较。青霉素敏感的S.在青霉素处理48小时内，戈登氏菌丧失>= 4 log（10）CFU/ml的活力。相比之下，Tol 1突变体损失= 4 log（10）CFU/ml，
Assessing bacterial viability by molecular markers might help accelerate the measurement of antibiotic-induced killing. This study investigated whether rRNA could be suitable for this purpose. Cultures of penicillin-susceptible and penicillin-tolerant (Tol1 mutant) Streptococcus gordonii were exposed to mechanistically different penicillin and levofloxacin. Bacterial survival was assessed by viable counts and compared to quantitative real-time PCR amplification of either the 16S rRNA genes or the 16S rRNA, following reverse transcription. Penicillin-susceptible S. gordonii lost >= 4 log(10) CFU/ml of viability over 48 h of penicillin treatment. In comparison, the Tol1 mutant lost = 4 log(10) CFU/ml and