Umbilical cord blood produces small megakaryocytes after transplantation
Umbilical cord blood produces small megakaryocytes after transplantation
复制标题
DOI：
10.1016/j.bbmt.2006.10.032
复制
发表时间：
2007-02-01
影响因子：
4.3
通讯作者：
Slayton, William B.
中科院分区：
文献类型：
--
作者：
Ignatz, Mark;Sola-Visner, Martha;Slayton, William B.
血小板植入延迟是脐带血移植的主要并发症。在体外，来自CB的巨核细胞小于来自骨髓（BM）或来自成人的动员外周血的巨核细胞。巨核细胞体积小可能导致血小板植入延迟。为了测试移植后小尺寸是否持续存在，我们测量了移植后获得的BM活检标本中的巨核细胞尺寸、浓度和成熟阶段，所述BM活检标本来自接受CB（CB组，n = 10）与动员的外周血或BM移植（BM组，n = 9）的患者的存档BM样品。CB组移植后BM样品中的巨核细胞明显小于BM组（中位直径，16.7 μ m vs 22.0 μ m）。CB组和BM组之间的巨核细胞浓度或成熟期无显著差异。我们第一次证明了人CB移植后CB衍生的巨核细胞达到成人大小的延迟。(c)2007年美国血液和骨髓移植学会。
Delayed platelet engraftment is a major complication of umbilical cord blood (CB) transplantation. Megakaryocytes derived from CB in vitro are smaller than megakaryocytes derived from bone marrow (BM) or mobilized peripheral blood from adults. Small megakaryocyte size may contribute to delayed platelet engraftment. To test whether small size persists after transplantation, we measured megakaryocyte size, concentration, and maturational stage in BM biopsy specimens obtained after transplantation in archived BM samples from patients receiving CB (CB group, n = 10) versus mobilized peripheral blood or BM transplantation (BM group, n = 9). Megakaryocytes in the postengraftment BM samples were significantly smaller in the CB group than in the BM group (median diameter, 16.7 vs 22.0 mu m). There were no significant differences in megakaryocyte concentration or maturational stage between the CB and BM groups. For the first time, we demonstrate that the attainment of adult size in CB-derived megakaryocytes is delayed after human CB transplantation. (c) 2007 American Society for Blood and Marrow Transplantation.