Impaired plasma nitric oxide availability and extracellular superoxide dismutase activity in healthy humans with advancing age
Impaired plasma nitric oxide availability and extracellular superoxide dismutase activity in healthy humans with advancing age
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DOI：
10.1016/j.lfs.2005.06.037
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发表时间：
2006-02-09
期刊：
影响因子：
6.1
通讯作者：
Ciancarelli, MGT
中科院分区：
文献类型：
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作者：
Di Massimo, C;Scarpelli, P;Ciancarelli, MGT
本研究旨在验证细胞外超氧化物歧化酶（EC-SOD）活性的改变及其在健康人群中随年龄增长而发生的血浆一氧化氮（NO）可用性损伤机制中的潜在参与。为此，从40名健康男性中抽取血浆样本，年龄在20-92岁，处于禁食状态，用于测量稳定的终产物亚硝酸盐/硝酸盐（NOx），作为NO可用性的表达，EC-SOD活性，硫代巴比妥酸反应物质（TBARS）作为脂质过氧化的标志，Trolox等效抗氧化能力（TEAC）作为血浆总抗氧化能力的衡量，以及低密度脂蛋白（LDL）对铜介导氧化的体外敏感性。评估为滞后时间。结果表明，年龄的增长与血浆NOx （r = -0.877, P < 0.001）、EC-SOD活性（r = -0.888, P < 0.001）、TEAC （r = -0.647, P < 0.001）和滞后时间（r = -0.621, P < 0.001）的降低以及血浆TBARS （r = 0.858, P < 0.001）的增加显著相关。血浆NO水平由EC-SOD活性和年龄独立预测。EC-SOD活性则由年龄和TEAC决定。综上所述，本研究的发现进一步揭示了与年龄相关的内皮功能障碍相关的机制，表明EC-SOD活性的下降可能通过与年龄相关的氧化/抗氧化平衡损害参与了随着年龄增长血浆NO可用性的逐渐降低。(c) 2005爱思唯尔公司版权所有。
This study is aimed to verify the modifications of extracellular superoxide dismutase (EC-SOD) activity and its potential involvement on the mechanism responsible for the impairment of plasma nitric oxide (NO) availability occurring with advancing age in healthy humans. For this purpose, plasma samples were drawn from 40 healthy men, aged 20-92 years, in fasting state and used for measurements of stable end-product nitrite/nitrate (NOx), as expression of NO availability, EC-SOD activity, thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation, Trolox equivalent antioxidant capacity (TEAC) as a measure of plasma total antioxidant capacity, and in vitro susceptibility of low density lipoprotein (LDL) to copper-mediated oxidation, evaluated as lag time. As indicated by our results, advancing age was significantly related to decreased plasma values of NOx (r = -0.877, P < 0.001), EC-SOD activity (r = -0.888, P < 0.001), TEAC (r = -0.647, P < 0.001) and lag time (r = -0.621, P < 0.001) as well as to an increased plasma amount of TBARS (r = 0.858, P < 0.001). NO, plasma level resulted independently predicted by EC-SOD activity and age. EC-SOD activity, in turn, was determined by age and TEAC. Taken together, findings of the present study give further insight into the mechanism related to age-associated endothelial dysfunction, indicating that the decreased EC-SOD activity may be involved in the progressive reduction of plasma NO availability with advancing age through the age-related impairment of oxidant/antioxidant balance. (c) 2005 Elsevier Inc. All rights reserved.