Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate.

Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate.
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通过核酸酶 P1 水解产物的高效液相色谱法测定 DNA 的碱基组成。

DOI:
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发表时间:
1988
期刊:
Nucleic acids symposium series
影响因子:
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通讯作者:
A. Kuninaka
A. Kuninaka
中科院分区:
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文献类型:
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作者:
M. Kumagai;M. Fujimoto;A. Kuninaka

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DNA 的碱基组成通过其核酸酶 P1 水解产物的高效液相色谱 (HPLC) 直接测定。即使DNA样品含有RNA或者样品量非常少,也可以令人满意地采用该方法。
The base composition of DNA was directly determined by high performance liquid chromatography (HPLC) of its nuclease P1 hydrolysate. This method can satisfactorily be employed even if the sample of DNA contains RNA or the amount of the sample is very small.