NEUTRALIZATION OF NEWCASTLE-DISEASE VIRUS BY MONOCLONAL-ANTIBODIES TO THE HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN - REQUIREMENT FOR ANTIBODIES TO 4 SITES FOR COMPLETE NEUTRALIZATION
NEUTRALIZATION OF NEWCASTLE-DISEASE VIRUS BY MONOCLONAL-ANTIBODIES TO THE HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN - REQUIREMENT FOR ANTIBODIES TO 4 SITES FOR COMPLETE NEUTRALIZATION
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DOI：
10.1128/jvi.51.2.445-451.1984
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
BRATT, MA
中科院分区：
文献类型：
--
作者：
IORIO, RM;BRATT, MA
测定了针对新城疫病毒血凝素-神经氨酸酶糖蛋白（HN） 4个抗原位点的单克隆抗体的中和特性。每一种抗体的中和作用导致非中和病毒的持久部分（1-17%），其变化取决于所识别的HN位点，但不取决于抗体。需要在HN上的所有4个位点添加抗体，以获得与多克隆小鼠抗血清相当的中和水平。高持久分数不是由于病毒聚集、病毒库存中高水平的变体、使用不足的抗体、低抗体亲和力或使用鸡细胞作为宿主所特有的效应。在任何抗体留下的持久部分中加入兔抗小鼠Ig，可进一步降低传染性，通常可降低2倍。因此，一些病毒颗粒能够结合抗体，同时保持其传染性。讨论了这些发现对中和机制的影响。
The neutralizing characteristics of monoclonal antibodies directed to 4 antigenic sites on the hemagglutinin-neuraminidase glycoprotein (HN) of Newcastle disease virus were determined. Neutralization by each antibody resulted in a persistent fraction (1-17%) of nonneutralized virus which varied depending on the HN site recognized, but not on the antibody. The addition of antibodies to all 4 sites on the HN was required to give a level of neutralization comparable with that obtained with polyclonal mouse antiserum. The high persistent fractions were not due to viral aggregates, a high level of variants in the virus stock, the use of insufficient antibody, low antibody avidity or an effect peculiar to the use of the chicken cells as host. The addition of rabbit anti-mouse Ig to the persistent fraction left by any of the antibodies resulted in a further reduction in infectivity, often by as much as 2 logs. Thus, some viral particles are capable of binding antibody while retaining their infectivity. The implications of these findings to the mechanism of neutralization are discussed.