Structural studies of elastic fibre and microfibrillar proteins.
Structural studies of elastic fibre and microfibrillar proteins.
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DOI：
10.1016/j.mbplus.2021.100078
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发表时间：
2021-12
影响因子：
--
通讯作者：
Baldock C
中科院分区：
文献类型：
--
作者：
Singh M;Becker M;Godwin ARF;Baldock C
弹性组织的功能特性归因于其细胞外基质的组成，特别是细胞外的多结构域可延伸弹性纤维和微纤维蛋白的范围。这些蛋白质包括弹性蛋白、胶原蛋白、潜伏性TGFβ结合蛋白（LTBP）和胶原蛋白，它们的生物物理和生物化学性质不仅赋予基质结构完整性，而且在组织稳态的基础机制中发挥重要作用。到目前为止，关于这些分子的结构和分级组装的结构信息一直具有挑战性，并且由于翻译后修饰及其导致柔性的多结构域性质，分辨率受到限制，这共同导致构象和结构异质性。在这篇综述中，我们描述了一些在弹性纤维中发现的基质蛋白质和新出现的技术，可以揭示它们的结构和动力学特性。
Elastic tissues owe their functional properties to the composition of their extracellular matrices, particularly the range of extracellular, multidomain extensible elastic fibre and microfibrillar proteins. These proteins include elastin, fibrillin, latent TGFβ binding proteins (LTBPs) and collagens, where their biophysical and biochemical properties not only give the matrix structural integrity, but also play a vital role in the mechanisms that underlie tissue homeostasis. Thus far structural information regarding the structure and hierarchical assembly of these molecules has been challenging and the resolution has been limited due to post-translational modification and their multidomain nature leading to flexibility, which together result in conformational and structural heterogeneity. In this review, we describe some of the matrix proteins found in elastic fibres and the new emerging techniques that can shed light on their structure and dynamic properties.