Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology
Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology
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DOI：
10.1016/j.envres.2020.110092
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发表时间：
2020-12-01
影响因子：
8.3
通讯作者：
Bivins, Aaron
中科院分区：
文献类型：
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作者：
Ahmed, Warish;Bertsch, Paul M.;Bivins, Aaron
基于废水的流行病学 (WBE) 展示了 COVID-19 社区传播监测的潜力；然而，需要废水中 SARS-CoV-2 RNA 稳定性的数据来解释 WBE 结果。通过逆转录定量聚合酶链反应 (RT-qPCR)，研究了未经处理的废水、高压灭菌废水和储存在 4、15、25 和 37 摄氏度的脱氯自来水中 SARS-CoV-2 和潜在替代物鼠肝炎病毒 (MHV) 的 RNA 衰减率。温度影响最大，其次是基质类型 SARS-CoV-2 RNA 一级衰减率 (k)。 SARS-CoV-2 RNA 的平均 T-90（减少 1-log(10) 所需的时间）在未经处理的废水中为 8.04 至 27.8 天，在高压灭菌废水中为 5.71 至 43.2 天，在自来水中为 9.40 至 58.6 天。 MHV RNA 在 4 至 37 摄氏度下的平均 T-90 在未经处理的废水中为 7.44 至 56.6 天，在高压灭菌废水中为 5.58-43.1 天，在自来水中为 10.9 至 43.9 天。 SARS-CoV-2和MHV的RNA衰减没有统计学上的显着差异；因此，建议将 MHV 作为合适的持久性替代品。两种病毒 RNA 在所有基质中的所有温度下的衰减率常数都具有可比性，但未经处理的 SARS-CoV-2 废水除外，该废水对高温的敏感性较低。因此，SARS-CoV-2 RNA 可能会在未经处理的废水中持续存在足够长的时间，以便为 WBE 应用提供可靠的检测。
Wastewater-based epidemiology (WBE) demonstrates potential for COVID-19 community transmission monitoring; however, data on the stability of SARS-CoV-2 RNA in wastewater are needed to interpret WBE results. The decay rates of RNA from SARS-CoV-2 and a potential surrogate, murine hepatitis virus (MHV), were investigated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in untreated wastewater, autoclaved wastewater, and dechlorinated tap water stored at 4, 15, 25, and 37 degrees C. Temperature, followed by matrix type, most greatly influenced SARS-CoV-2 RNA first-order decay rates (k). The average T-90 (time required for 1-log(10) reduction) of SARS-CoV-2 RNA ranged from 8.04 to 27.8 days in untreated wastewater, 5.71 to 43.2 days in autoclaved wastewater, and 9.40 to 58.6 days in tap water. The average T-90 for RNA of MHV at 4 to 37 degrees C ranged from 7.44 to 56.6 days in untreated wastewater, 5.58-43.1 days in autoclaved wastewater, and 10.9 to 43.9 days in tap water. There was no statistically significant difference between RNA decay of SARS-CoV-2 and MHV; thus, MHV is suggested as a suitable persistence surrogate. Decay rate constants for all temperatures were comparable across all matrices for both viral RNAs, except in untreated wastewater for SARS-CoV-2, which showed less sensitivity to elevated temperatures. Therefore, SARS-CoV-2 RNA is likely to persist long enough in untreated wastewater to permit reliable detection for WBE application.