Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination
Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination
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DOI：
10.1016/j.phymed.2011.05.009
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发表时间：
2011-09-15
期刊：
影响因子：
7.9
通讯作者：
Dicko, M. H.
中科院分区：
文献类型：
--
作者：
Bassole, I. H. N.;Lamien-Meda, A.;Dicko, M. H.
为研究布基纳法索植物精油的化学成分和抗菌特性，采用气相色谱- fid和气相色谱-质谱联用技术对产自布基纳法索的Cymbopogon citratus和Cymbopogon giganteus的精油进行了分析。在柑橘精油中鉴定出5种成分，占挥发油的96.3%。主要成分为香樟（48.1%）、梧桐（34.6%）和月桂烯（11.0%）。从巨角草中鉴定出8种化合物，占总挥发油的86.0%。主要化合物为柠檬烯（42%）和一组单萜醇：反式对薄荷-1(7)，8-二烯-2-醇（14.2%），顺式对薄荷-1(7)，8-二烯-2-醇（12%），反式对薄荷-2,8-二烯-1-醇（5.6%）和顺式对薄荷-2,8-二烯-1-醇（5.2%）。采用圆盘扩散法和微量稀释法对9种细菌进行抑菌试验。巨孢葡萄球菌对所有微生物均有抑菌作用，而柑橘葡萄球菌对铜绿假单胞菌无抑菌作用。采用棋盘法定量测定两种EOs组合的抑菌活性。两种EOs联合使用具有协同、加性和无差异的抗菌作用。本研究的结果提供了证据，表明可以评估植物EOs组合的协同活性，以降低其最小有效剂量。(C) 2011爱思唯尔有限公司版权所有。
As part of ongoing research on the chemical composition and the antimicrobial properties of Burkinabe plants essential oils alone and in combination, essential oils (EOs) from leaves of Cymbopogon citratus and Cymbopogon giganteus from Burkina Faso were analyzed by GC-FID and GC-MS. Five constituents, which accounted for 96.3% of the oil, were identified in the EO of C citratus. Geranial (48.1%), neral (34.6%) and myrcene (11.0%) were the major constituents. For C. giganteus a total of eight compounds were identified which represented 86.0% of the oils extracted. The dominant compounds were limonene (42%) and a set of monoterpene alcohols: trans-p-mentha-1(7),8-dien-2-ol (14.2%), cis-p-mentha-1(7),8-dien-2-ol (12%), trans-p-mentha-2,8-dien-1-ol (5.6%) and cis-p-mentha-2,8-dien-1-ol (5.2%). The EOs were tested against nine bacteria by using disc diffusion and microdilution methods. C giganteus EO showed antimicrobial effects against all microorganisms tested whereas C. citratus EO failed to inhibit Pseudomonas aeruginosa. The antimicrobial activity of combinations of the two EOs was quantified by the checkerboard method. Combinations of the two EOs exerted synergistic, additive and indifferent antimicrobial effects. Results of the present investigation provide evidence that the combinations of plant EOs could be assessed for synergistic activity in order to reduce their minimum effective dose. (C) 2011 Elsevier GmbH. All rights reserved.