The large scale purification of ubiquitin from human erythrocytes.
The large scale purification of ubiquitin from human erythrocytes.
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DOI：
10.1080/00327488508062433
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
A. Haas;K. Wilkinson
中科院分区：
文献类型：
--
作者：
A. Haas;K. Wilkinson
描述了一种从人红细胞中大规模纯化活性泛素的简单、可重复的方法。红细胞每立方厘米浓缩细胞含有 100 微克游离泛素，其中 44% 可以通过热处理、硫酸铵分级分离和离子交换层析的组合以均质形式回收。
A simple, reproducible method for the large-scale purification of active ubiquitin from human erythrocytes is described. Erythrocytes contain 100 micrograms free ubiquitin per cc of packed cells, of which 44% can be recovered in homogeneous form by a combination of heat treatment, ammonium sulfate fractionation, and ion exchange chromatography.