Harnessing natural transformation in Actinobacillus pleuropneumoniae:: a simple method for allelic replacements
Harnessing natural transformation in Actinobacillus pleuropneumoniae:: a simple method for allelic replacements
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DOI：
10.1111/j.1574-6968.2004.tb09492.x
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发表时间：
2004-04-15
影响因子：
2.1
通讯作者：
Langford, PR
中科院分区：
文献类型：
--
作者：
Bossé, JT;Nash, JHE;Langford, PR
我们研究了使用自然转化方案将突变引入胸膜肺炎放线杆菌血清型1和5 b。对于测试的两种菌株，我们在丰富培养基中培养期间回收了1/10（8）的转化体，该结果与生长期无关。这种低频率的A.当细菌在已知最适合诱导流感嗜血杆菌感受态的条件下生长时，胸膜肺炎没有增加。将含有卡那霉素盒的线性化质粒DNA插入A.在胸膜肺炎血清型1中，我们表明自然转化可以用作将等位基因置换引入该细菌的简单方法，并且可以用于将突变从一种血清型转移到另一种血清型。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
We have investigated the use of a natural transformation protocol to introduce mutations into Actinobacillus pleuropneumoniae serotypes 1 and 5b. For both strains tested, we recovered 1 in 10(8) transformants during culture in rich medium, a result that was independent of the growth phase. This low frequency of transformation of A. pleuropneumoniae did not increase when bacteria were grown under conditions known to be optimal for induction of competence in Haemophilus influenzae. Using linearised plasmid DNA containing a kanamycin cassette inserted into the sodC gene of A. pleuropneumoniae serotype 1, we showed that natural transformation can be used as a simple method for introducing allele replacements into this bacterium, and can be used to transfer mutations from one serotype to another. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.