Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation
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DOI：
10.1073/pnas.0606571103
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发表时间：
2006-09-26
影响因子：
11.1
通讯作者：
Furukubo-Tokunaga, Katsuo
中科院分区：
文献类型：
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作者：
Kobayashi, Masatomo;Michaut, Lydia;Furukubo-Tokunaga, Katsuo
蘑菇体（MB）是果蝇大脑中嗅觉联想学习和基本认知功能的中心。作为系统阐明在 MB 中优先表达的基因的一种方法，我们分析了与羟基脲消除 MB 相关的转录谱的全基因组变化。我们根据微阵列数据选择了 100 个基因，并通过原位杂交检查了它们在大脑中的表达模式。 70 个基因被发现在后背皮质中表达，该皮质包含 MB 细胞体。这些基因编码具有多种功能的蛋白质，包括转录、信号传导、细胞粘附、通道和转运蛋白。此外，我们还通过转基因RNA干扰检查了40个微阵列鉴定的基因的发育功能；当用 MB-Gal4 驱动程序抑制时，发现 8 个基因会导致轻度至重度的 MB 缺陷。这些结果不仅提供了关于控制MB发育的基因库的重要信息，而且还提供了可能在MB可塑性中具有重要生理功能的神经因子库的重要信息。
Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation by hydroxyurea. We selected 100 genes based on microarray data and examined their expression patterns in the brain by in situ hybridization. Seventy genes were found to be expressed in the posterodorsal cortex, which harbors the MB cell bodies. These genes encode proteins of diverse functions, including transcription, signaling, cell adhesion, channels, and transporters. Moreover, we have examined developmental functions of 40 of the microarray-identified genes by transgenic RNA interference; 8 genes were found to cause mild-to-strong MB defects when suppressed with a MB-Gal4 driver. These results provide important information not only on the repertoire of genes that control MB development but also on the repertoire of neural factors that may have important physiological functions in MB plasticity.