The chemopreventive flavonoid apigenin confers radiosensitizing effect in human tumor cells grown as monolayers and spheroids.
The chemopreventive flavonoid apigenin confers radiosensitizing effect in human tumor cells grown as monolayers and spheroids.
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DOI：
10.1269/jrr.0635
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发表时间：
2007
影响因子：
2
通讯作者：
N. Watanabe;R. Hirayama;N. Kubota
中科院分区：
文献类型：
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作者：
N. Watanabe;R. Hirayama;N. Kubota
芹菜素是一种常见的膳食类黄酮，存在于许多水果和蔬菜中，是一种非诱变化学预防剂。在本研究中，我们研究了芹菜素对源自人肺癌的 SQ-5 细胞放射敏感性的影响。将活跃生长的细胞在含有 40 muM 芹菜素的培养基中于 37°C 孵育 16 小时。然后用X射线照射细胞并与芹菜素一起孵育另外8小时。使用克隆形成测定来评估放射敏感性。使用吖啶橙/溴化乙锭双染色评估细胞凋亡和坏死。与未与芹菜素一起孵育的细胞相比，与芹菜素一起孵育的细胞表现出显着更高的放射敏感性和凋亡水平。通过蛋白质印迹法测量蛋白质水平。与芹菜素一起孵育会增加 WAF1/p21 的蛋白表达并降低 Bcl-2 的蛋白表达。此外，芹菜素使 SQ-5 球体（以三维结构生长的细胞聚集体，模拟体内肿瘤的生长和微环境条件）对辐射敏感。因此，芹菜素似乎是一种有前途的用于对抗人类癌症的放射增敏剂。
Apigenin, a common dietary flavonoid present in many fruits and vegetables, is a nonmutagenic chemopreventive agent. In the present study, we investigated the effect of apigenin on the radiosensitivity of SQ-5 cells, which are derived from a human lung carcinoma. Actively growing cells were incubated for 16 h at 37 degrees C in medium containing 40 muM apigenin. The cells were then irradiated with X-rays and incubated with apigenin for a further 8 h. Radiosensitivity was assessed using a clonogenic assay. Apoptosis and necrosis were assessed using acridine orange/ethidium bromide double staining. Cells incubated with apigenin exhibited significantly greater radiosensitivity and apoptosis levels than cells not incubated with apigenin. Protein levels were measured by Western blotting. Incubation with apigenin increased protein expression of WAF1/p21 and decreased protein expression of Bcl-2. Furthermore, apigenin sensitized SQ-5 spheroids (cell aggregates growing in a three-dimensional structure that simulate the growth and microenvironmental conditions of in vivo tumors) to radiation. Thus, apigenin appears to be a promising radiosensitizing agent for use against human carcinomas.