A novel X-linked member of the human zinc finger protein gene family: isolation, mapping, and expression.
A novel X-linked member of the human zinc finger protein gene family: isolation, mapping, and expression.
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人类锌指蛋白基因家族的新 X 连锁成员：分离、作图和表达。
DOI：
10.1007/bf00417431
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Grimaldi,G
中科院分区：
文献类型：
--
作者：
Marino,M;Archidiacono,N;Franzé,A;Rosati,M;Rocchi,M;Ballabio,A;Grimaldi,G
我们报告了从 X 染色体 (Chr) 特异性文库中分离出的 Krüppel 型新型推定锌指基因 (ZNF81) 的部分特征。人-仓鼠体细胞杂交体中与 ZNF81 指结构域同源的序列的分离模式已确定它位于 Xp22.1-Xp11 区域内。 ZNF81 与 ZFX、ZNF41 和 ZNF21 一起代表了位于人类 X Chr 短臂内的锌指基因家族成员的另一个例子。序列分析显示ZNF81可能编码在C末端含有Krüppel型12个典型C2H2锌指串联阵列的多肽。 Northern 分析表明，来自 ZNF81 指结构域的探针与多种细胞系中存在的聚腺苷酸化转录物杂交，这一结果支持了其是该多基因家族的表达的功能性成员的假设。
We report the partial characterization of a novel putative zinc finger gene of theKrüppel-type (ZNF81), isolated from an X Chromosome (Chr) specific library. The pattern of segregation in human-hamster somatic cell hybrids of sequences homologous to the ZNF81 finger domain has established that it resides within the Xp22.1-Xp11 region. ZNF81 represents yet another example, together with ZFX, ZNF41, and ZNF21, of members of the zinc finger gene family residing within the short arm of the human X Chr. Sequence analysis showed that ZNF81 may encode a polypeptide(s) containing tandem arrays of 12 canonical C2H2zinc fingers of theKrüppel-type at the C-terminus. Northern analysis indicated that probes from the ZNF81 finger domain hybridize to polyadenylated transcripts present in several cell lines, a result that supports the hypothesis that it is an expressed, functional member of this multigene family.