Sfl1p acts as an activator of the HSP30 gene in Saccharomyces cerevisiae
Sfl1p acts as an activator of the HSP30 gene in Saccharomyces cerevisiae
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DOI：
10.1007/s00294-007-0136-z
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发表时间：
2007-08-01
期刊：
影响因子：
2.5
通讯作者：
Blondin, Bruno
中科院分区：
文献类型：
--
作者：
Galeote, Virginie Ansanay;Alexandre, Herve;Blondin, Bruno
在酵母中，已知环境挑战诱导特异性和一般应激反应。当细胞暴露于各种应激时，HSP 30基因被强烈诱导，但这种激活在很大程度上独立于主要的应激相关转录因子Hsf 1 p，部分独立于Msn 2 p/Msn 4p。为了确定新的潜在的调节HSP 30，我们分离的插入突变体影响HSP 30的表达。我们确定了SFL 1基因编码的蛋白质以前显示抑制几个基因。我们表明，Sfl 1参与了HSP 30的转录激活。sfl 1突变降低HSP 30-lacZ表达的基础和应力诱导的条件下。我们还表明，使用定点突变，HSL图案（热休克样推定的DNA结合序列）位于热休克蛋白30启动子所需的热休克蛋白30激活。最后，对SFL 1缺失对基因表达影响的全基因组分析显示，Sfl 1 p控制着少数基因的表达，其中一些基因被蛋白质激活，另一些基因被抑制。作为一个整体，我们的数据表明，Sfl 1 p是应激反应基因HSP 30的转录控制的关键组成部分。此外，我们表明，Sfl 1，这是以前被描述为一个转录抑制因子，也可以作为一个激活剂。
In the yeast, environmental challenges are known to induce both specific and general stress response. The HSP30 gene is strongly induced when cells are exposed to various stresses but this activation is largely independent of the major stress-related transcription factor Hsf1p and partly independent from Msn2p/Msn4p. In order to identify new potential regulators of HSP30 we isolated insertion mutants affected in HSP30 expression. We identified SFL1 gene encoding a protein previously shown to repress several genes. We show that Sfl1 is involved in the transcriptional activation of HSP30. Mutation of sfl1 reduces HSP30-lacZ expression under both basal and stress-induced conditions. We also show, using site-directed mutagenesis, that HSL motifs (Heat-Shock-Like putative DNA binding sequence) located in HSP30 promoter are required for HSP30 activation. Finally, a genome-wide analysis of the effects of SFL1 deletion on gene expression revealed that Sfl1p controls the expression of a small number of genes, with some being activated by the protein and others repressed. As a whole our data show that Sfl1p is a key component of the transcriptional control of the stress responsive gene HSP30. Moreover, we show that Sfl1, which was previously described as being a transcriptional repressor, can also act as an activator.