ELEVATED EXPRESSION OF THE RIBOSOMAL PROTEIN-S2 GENE IN HUMAN TUMORS
ELEVATED EXPRESSION OF THE RIBOSOMAL PROTEIN-S2 GENE IN HUMAN TUMORS
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DOI：
10.1002/mc.2940050309
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发表时间：
1992-01-01
影响因子：
4.6
通讯作者：
TAINSKY, MA
中科院分区：
文献类型：
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作者：
CHIAO, PJ;SHIN, DM;TAINSKY, MA
cDNA文库的差异筛选用于分离在非致瘤性克隆和人畸胎瘤细胞系PA-1的ras转化变体中差异表达的基因。我们鉴定的一个cDNA克隆的RNA转录本在ras转化的PA-1细胞中的表达水平比在非致瘤性PA-1细胞中高25倍。该克隆的DNA序列分析表明，它与小鼠LLRep 3基因具有86%的核酸同源性，并且仅在单个氨基酸密码子（密码子198）处不同，该密码子从LLRep 3中的丝氨酸变为该cDNA克隆中的苏氨酸。大鼠核糖体S2蛋白与酵母全能信息抑制子SUP 44密切相关，该抑制子编码酵母核糖体蛋白S4;与小鼠蛋白LLRep 3密切相关;与我们在本报告中描述的人cDNA克隆密切相关。因此，我们得出结论，该克隆编码人类核糖体S2蛋白。原位杂交实验表明，该基因的表达升高培养的人头颈部鳞状细胞癌与正常角质形成细胞相比。原位杂交实验还表明，该基因的表达升高，在人类癌前白斑，头颈部鳞状细胞癌，结肠癌和乳腺癌的组织切片与邻近的正常组织相比。S2表达可能是一个有用的诊断或预后指标，用于人类肿瘤的分级。
Differential screening of a cDNA library was used to isolate genes differentially expressed in a nontumorigenic clone and a ras-transformed variant of the human teratocarcinoma cell line PA-1. The RNA transcript for one of the cDNA clones that we identified was expressed at a 25-fold higher level in the ras-transformed PA-1 cells than in the nontumorigenic PA-1 cells. DNA sequence analysis of this clone showed that it had 86% nucleic acid homology to the mouse LLRep3 gene and only differed at a single amino acid codon (codon 198), which is changed from serine in LLRep3 to threonine in this cDNA clone. The rat ribosomal S2 protein is closely related to the yeast omnipotent informational suppressor SUP44, which encodes the yeast ribosomal protein S4; to the mouse protein LLRep3; and to the human cDNA clone we describe in this report. We therefore concluded that this clone codes for the human ribosomal S2 protein. In situ hybridization experiments revealed that expression of this gene was elevated in cultured human head and neck squamous cell carcinomas compared with normal keratinocytes. In situ hybridization experiments also demonstrated that expression of this gene was elevated in histological sections of human premalignant leukoplakia, head and neck squamous cell carcinomas, and colon and breast cancers compared with the adjacent normal tissues. S2 expression may be a useful diagnostic or prognostic marker for grading human tumors.