Optimization of a mouse locomotor rating system to evaluate compression-induced spinal cord injury: correlation of locomotor and morphological injury indices
Optimization of a mouse locomotor rating system to evaluate compression-induced spinal cord injury: correlation of locomotor and morphological injury indices
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DOI：
10.3171/spi.2006.4.2.165
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发表时间：
2006-02-01
影响因子：
2.8
通讯作者：
Linden, J
中科院分区：
文献类型：
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作者：
Li, JS;Oskouian, RJ;Linden, J
Object.由于小鼠遗传学的有用性，需要改进用于产生和评估小鼠脊髓损伤（SCI）的程序。作者描述了一种改进的运动评分系统，用于评估SCI。将改良的Basso-Beattie-Bresnahan（mBBB）评分系统与现有程序以及组织学SCI标准进行比较。通过在椎板切除术后在T-12处将重物放置在脊髓上5至15分钟以产生脊髓缺血来使小鼠经受SCI。使用mBBB评分评估损伤，该评分结合了最适合精确评估小鼠SCI的大鼠BBB和小鼠运动功能评分系统的元素。发现mBBB评分比斜面试验更具鉴别力，并且在作者的实验室中，其变异系数显著低于Basso小鼠量表评分。mBBB评分与髓鞘的铬花青染色评估的白色物质的保留良好相关。在小鼠T-12的重量放置导致可再现的SCI。一种新的mBBB评分系统可用于准确评估小鼠SCI后的运动功能障碍，并与脊髓白色物质的组织学评估密切相关。
Object. Due to the usefulness of mouse genetics, there is a need to improve procedures for producing and assessing spinal cord injury (SCI) in mice. The authors describe an improved locomotor scoring system for evaluating SCI. The modified Basso-Beattie-Bresnahan (mBBB) scoring system for mice is compared with existing procedures as well as histological SCI criteria.Methods. Mice were subjected to SCI by placing a weight on the cord at T-12 for 5 to 15 minutes after laminectomy to produce spinal cord ischemia. Injury was assessed using mBBB scoring that incorporates elements of the rat BBB and the mouse motor function scoring systems that are best suited for precisely assessing mouse SCI. The mBBB score was found to be more discriminating than the inclined plane test, and in the authors' laboratory it had a significantly lower coefficient of variation than the Basso mouse scale score. The mBBB score is well correlated with sparing of white matter as assessed by eriochrome cyanine staining of myelin.Conclusions. Weight placement at T-12 in the mouse causes reproducible SCI. A new mBBB scoring system is useful for accurately assessing locomotor dysfunction following SCI in mice and is well correlated with histological assessment of spinal cord white matter.