A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI
A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI
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DOI：
10.1007/bf01569654
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
HARTMAN, PA
中科院分区：
文献类型：
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作者：
HOLT, SM;HARTMAN, PA
从枯草芽孢杆菌（Bacillussubtilis）中纯化的内切葡聚糖酶（Eg）与疣孢漆斑菌（Myrotheciumverrucaria）和里氏木霉（Trichodermareesei）的Eg粗品在SDS存在下热变性，并用SDS聚丙烯酰胺凝胶电泳分离。 酶复性后，在羧甲基纤维素分离胶上直接检测酶谱活性条带。 变性酶谱分析是一种简便的分离检测Egs的方法。
A purified endoglucanase (Eg) from Bacillus subtilis and crude Eg preparations from Myrothecium verrucaria and Trichoderma reesei were denatured by heat in the presence of SDS and separated by using SDS polyacrylamide gel electrophoresis. Eg zymogram activity bands were detected directly in carboxymethylcellulose-laden separating gels after enzyme renaturation. Denaturing zymogram analysis was a simple method for the separation and detection of Egs.