Peri-Implantitis versus Periodontitis: Functional Differences Indicated by Transcriptome Profiling
Peri-Implantitis versus Periodontitis: Functional Differences Indicated by Transcriptome Profiling
复制标题
DOI：
10.1111/cid.12001
复制
发表时间：
2014-06-01
影响因子：
3.6
通讯作者：
Haesler, Robert
中科院分区：
文献类型：
--
作者：
Becker, Stephan T.;Beck-Broichsitter, Benedicta E.;Haesler, Robert
背景 牙周炎和种植体周围炎经常被作为一个整体来讨论，这在治疗方法和科学方法中都有体现。目前尚不清楚临床特征的相似性在多大程度上归因于潜在疾病机制的相似性。目的 该研究的主要目的是显示种植体周围炎和牙周炎在 mRNA 水平上是否存在差异或有何差异，代表疾病相关事件的高分辨率图谱。材料和方法 为了描述体内的病理生理机制，使用了来自 7 名种植体周围炎患者、7 名牙周炎患者和 8 名健康对照的原发牙龈组织，以生成全基因组转录组谱。结果基于定量转录组分析，我们可以发现种植体周围炎和牙周炎表现出显着不同的 mRNA 特征。此外，我们还提出了与疾病相关的 mRNA 谱，它显示了潜在的种植体周围炎疾病机制。基因本体分析揭示了多种途径，支持种植体周围炎是一种具有独特病理生理学的复杂炎症性疾病的假设。在种植体周围炎组织中，与先天免疫反应和防御反应相关的转录物调节占主导地位，而在牙周炎组织中，细菌反应系统占主导地位。结论 综上所述，我们的结果表明，将种植体周围炎和牙周炎视为具有共同特征和独特特征的疾病实体，这应该在治疗和科学层面上得到体现。
Background Periodontitis and Periimplantitis are oftentimes discussed as one entity, which is reflected by therapeutical as well as by scientific approaches. It is unclear, to which extent the similarity of the clinical characteristics is attributed to similarities in the underlying disease mechanisms. Purpose The main objective of the study is to display if or how different periimplantitis and periodontitis are on the mRNA level, representing a high-resolution map of disease-associated events. Materials and Methods Aiming to describe the pathophysiological mechanisms in vivo, primary gingival tissue from 7 periimplantitis patients, 7 periodontitis patients and 8 healthy controls was employed in order to generate genome wide transcriptome profiles. Results On the basis of quantitative transcriptome analysis, we could show that periimplantitis and periodontitis exhibit significantly different mRNA signatures. Additionally we present a disease associated mRNA profile, which displays potential periimplantitis disease mechanisms. A gene ontology analysis revealed various pathways, supporting the hypothesis of periimplantitis being a complex inflammatory disorder with a unique pathophysiology. While in periimplantitis tissue the regulation of transcripts related to innate immune responses and defense responses were dominating, in periodontitis tissues bacterial response systems prevailed. Conclusions Taken together, our results suggest considering periimplantitis and periodontitis as disease entities with shared as well as with distinct features, which should be reflected on the therapeutical as well as on the scientific level.