Src acts with WNT/FGFRL signaling to pattern the planarian anteroposterior axis
Src acts with WNT/FGFRL signaling to pattern the planarian anteroposterior axis
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DOI：
10.1242/dev.200125
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发表时间：
2022-04-01
期刊：
影响因子：
4.6
通讯作者：
Petersen, Christian P.
中科院分区：
文献类型：
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作者：
Bonar, Nicolle A.;Gittin, David, I;Petersen, Christian P.
组织身份的确定是至关重要的再生，和Planarian前后（AP）轴使用位置控制基因表达的体壁肌肉，以确定身体的区域化。经典的Wnt信号通过notum和wnt 1信号建立了前极和后极的身份，两条Wnt/FGFRL信号通路控制头部和躯干结构域，但其下游信号机制尚未完全了解。在这里，我们确定了一个Planarian Src同系物，限制头部和躯干身份的前位置。src-1（RNAi）动物形成了增大的脑和异位眼，并且还复制了躯干组织，类似于Wnt/FGFRL RNAi表型的组合。src-1是建立施密茶中位置控制基因表达的区域所必需的，表明它在AP轴图案化的上游步骤起作用。双RNAi实验和眼睛再生测定表明src-1可以与至少一些Wnt和FGFRL因子平行作用。src-1与其他后促进因子的共抑制导致了戏剧性的模式变化和Wnt/FGFRL的重编程控制新的位置输出。这些结果将src-1鉴定为促进AP位置系统的鲁棒性的因素，该AP位置系统指示适当的再生。
Tissue identity determination is crucial for regeneration, and the planarian anteroposterior (AP) axis uses positional control genes expressed from body wall muscle to determine body regionalization. Canonical Wnt signaling establishes anterior versus posterior pole identities through notum and wnt1 signaling, and two Wnt/FGFRL signaling pathways control head and trunk domains, but their downstream signaling mechanisms are not fully understood. Here, we identify a planarian Src homolog that restricts head and trunk identities to anterior positions. src-1(RNAi) animals formed enlarged brains and ectopic eyes and also duplicated trunk tissue, similar to a combination of Wnt/FGFRL RNAi phenotypes. src-1 was required for establishing territories of positional control gene expression in Schmidtea mediterranea, indicating that it acts at an upstream step in patterning the AP axis. Double RNAi experiments and eye regeneration assays suggest src-1 can act in parallel to at least some Wnt and FGFRL factors. Co-inhibition of src-1 with other posterior-promoting factors led to dramatic patterning changes and a reprogramming of Wnt/FGFRLs into controlling new positional outputs. These results identify src-1 as a factor that promotes robustness of the AP positional system that instructs appropriate regeneration.