A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction.
A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction.
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DOI：
10.1089/ten.tec.2016.0250
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发表时间：
2016-11
影响因子：
3
通讯作者：
LiuWenlong;DanXiuli;WangTing;W. LuWilliam;PanHaobo
中科院分区：
文献类型：
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作者：
LiuWenlong;DanXiuli;WangTing;W. LuWilliam;PanHaobo
在设计新型生物材料的临床前和理论研究中，开发一种能够快速评估骨骼和骨科植入物之间相互作用的最佳动物模型是至关重要的。与其他动物模型相比，小鼠在获得发达的转基因修饰技术（如细胞示迹、敲除、敲入等）方面具有优势，这些技术是研究分子机制的有力工具。在这项研究中，我们建立了一个小鼠模型，专门用于评估骨-植入物在负重骨髓微环境中的相互作用，并有可能通过转基因技术研究生物材料的分子机制。详细记录了小鼠股骨干骺端骨缺损（Φ = 0.8 mm）的显微手术过程。根据我们的研究结果，一种经过充分研究的45S5生物活性材料的骨传导和骨整合特性。
The development of an optimal animal model that could provide fast assessments of the interaction between bone and orthopedic implants is essential for both preclinical and theoretical researches in the design of novel biomaterials. Compared with other animal models, mice have superiority in accessing the well-developed transgenic modification techniques (e.g., cell tracing, knockoff, knockin, and so on), which serve as powerful tools in studying molecular mechanisms. In this study, we introduced the establishment of a mouse model, which was specifically tailored for the assessment of bone–implant interaction in a load-bearing bone marrow microenvironment and could potentially allow the molecular mechanism study of biomaterials by using transgenic technologies. The detailed microsurgery procedures for developing a bone defect (Φ = 0.8 mm) at the metaphysis region of the mouse femur were recorded. According to our results, the osteoconductive and osseointegrative properties of a well-studied 45S5 bioactive...