Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae
Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae
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DOI：
10.1111/j.1399-302x.2007.00386.x
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发表时间：
2008-02-01
影响因子：
--
通讯作者：
Amano, A.
中科院分区：
文献类型：
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作者：
Inaba, H.;Nakano, K.;Amano, A.
背景/目标：牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周病病原菌，根据其菌毛fimA基因的多样性，将其菌毛分为I-V型和Ib型。累积的证据表明，具有II型菌毛的牙龈卟啉单胞菌菌株比具有其他类型的菌株更具毒性。然而，它是未知的，如果强大的毒力是一致的保守与II型菌毛的克隆。在本研究中，我们使用小鼠脓肿模型比较了具有II型菌毛的牙龈卟啉单胞菌临床分离株的感染性炎症变化，以检查其致病异质性和异质性相关因素。方法：将具有II型菌毛的9种不同临床分离株的悬液皮下注射到雌性BALB/c小鼠中，并比较炎性参数，如血清唾液酸浓度。许多II型菌毛分离株在小鼠中引起严重的炎症，尽管有些致病性较低，如对照菌株ATCC 33277（I型菌毛菌株）。这些结果表明，具有II型菌毛的牙龈卟啉单胞菌克隆之间存在致病异质性。进一步分析了牙龈卟啉单胞菌的异质性相关因素，发现牙龈卟啉单胞菌的致病潜力与牙龈卟啉单胞菌蛋白酶活性和侵袭效率呈正相关，而与疏水性和自聚集性无关。结论：致病性异质性与具有II型菌毛的牙龈卟啉单胞菌克隆的侵袭和蛋白水解活性有关。
Background/aims: Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. Accumulated evidence suggests that P. gingivalis strains with type II fimbriae are more virulent as compared to those with other types. However, it is unknown if strong virulence is uniformly conserved among clones with type II fimbriae. In the present study, we compared infectious inflammatory changes in clinical isolates of P. gingivalis with type II fimbriae using a mouse abscess model to examine their pathogenic heterogeneity and heterogeneity-related factors.Methods: Suspensions of nine different clinical isolates with type II fimbriae were subcutaneously injected into female BALB/c mice and inflammatory parameters, such as serum sialic acid concentration, were compared.Results: Many of the type II fimbrial isolates caused severe inflammation in the mice, though some were less causative, as was the control strain ATCC 33277 (type I fimbria strain). These results showed that pathogenic heterogeneity exists among P. gingivalis clones with type II fimbriae. Further, the heterogeneity-related factors of P. gingivalis strains were analyzed and the pathogenic potentials showed positive relationships to gingipain activities and invasive efficiency but not to hydrophobicity or autoaggregation. In addition, invasive efficiency was related to the activities of gingipains that were extracellularly secreted.Conclusion: These results suggest that pathogenic heterogeneity has relationships with the invasive and proteolytic activities of P. gingivalis clones with type II fimbriae.