Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion
Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion
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重新增殖的小胶质细胞仅源自急性耗竭后残留小胶质细胞的增殖
DOI：
10.1038/s41593-018-0090-8
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发表时间：
2018-04-01
影响因子：
25
通讯作者：
Peng, Bo
中科院分区：
文献类型：
--
作者：
Huang, Yubin;Xu, Zhen;Peng, Bo
新生的小胶质细胞在成年小鼠中选择性消除大多数小胶质细胞（>99%）后迅速补充整个大脑。先前的研究报道，再生的小胶质细胞主要来源于脑中表达巢蛋白的小胶质祖细胞。然而，这些重新填充的小胶质细胞的起源一直存在激烈的争论。在这项研究中，我们调查的起源，重新填充小胶质细胞的一系列的命运映射的方法。我们首先通过联体共生排除了重新填充的小胶质细胞的血液来源。使用不同的转基因小鼠品系，我们证明了所有重新增殖的小胶质细胞都来自少数存活的小胶质细胞（<1%）的增殖。尽管在新形成的小胶质细胞中巢蛋白表达的短暂模式，但没有一个重新填充的小胶质细胞来自巢蛋白阳性的非小胶质细胞。总之，我们的结论是，重新填充的小胶质细胞完全来自残留的小胶质细胞，而不是从头祖细胞，这表明在成人大脑中的小胶质细胞祖细胞的情况下。
Newborn microglia rapidly replenish the whole brain after selective elimination of most microglia (>99%) in adult mice. Previous studies reported that repopulated microglia were largely derived from microglial progenitor cells expressing nestin in the brain. However, the origin of these repopulated microglia has been hotly debated. In this study, we investigated the origin of repopulated microglia by a series of fate-mapping approaches. We first excluded the blood origin of repopulated microglia via parabiosis. With different transgenic mouse lines, we then demonstrated that all repopulated microglia were derived from the proliferation of the few surviving microglia (<1%). Despite a transient pattern of nestin expression in newly forming microglia, none of repopulated microglia were derived from nestin-positive non-microglial cells. In summary, we conclude that repopulated microglia are solely derived from residual microglia rather than de novo progenitors, suggesting the absence of microglial progenitor cells in the adult brain.