Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and Their Applications for Use
Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and Their Applications for Use
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凝集素型泛素连接酶亚基：Fbs 蛋白及其应用
DOI：
10.1007/978-1-0716-0430-4_22
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yukiko Yoshida
中科院分区：
文献类型：
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作者：
楼 希超;津釜 大侑;グプタ ク マル;高野 哲夫;船戸耕一;Yukiko Yoshida
在哺乳动物中发现了三种凝集素类型的F-box蛋白，称为F-box蛋白识别糖链(FBS)，它们是SCF(Skp1/Cullin1/F-box蛋白)复合泛素连接酶的底物结合亚基。SCFFbs识别胞浆N-葡聚糖为不利细胞状态的信号，并泛素化出现在胞浆中的糖蛋白以将其从细胞中移除。虽然FBS蛋白识别最内部的Man3GlcNAc2结构，这种结构通常存在于大多数N-糖链结构中，但它们优先结合高甘露糖型糖链。最近，重组Fbs1衍生蛋白已被开发为一种全面浓缩N-糖肽的工具。标记的Fbs3也可用作检测细胞中细胞器损伤的工具，因为它具有使其快速积累在受损细胞器中的特性。在这一章中，我们介绍了FBS蛋白的两个应用：无偏见的N-糖肽捕获方法和检测活细胞中受损的细胞器。
Three lectin-type F-box proteins called Fbs (F-box protein-recognizingsugar chains) are found in mammals, and function as substrate-binding subunits in the SCF (Skp1/Cullin1/F-box protein) complex ubiquitin ligases. The SCFFbsrecognizes cytosolicN-glycans as a signal for an adverse cellular state, and ubiquitinates glycoproteins which appear in the cytosol to remove them from cells. Although Fbs proteins recognize innermost Man3GlcNAc2structure that is commonly found in mostN-glycan structures, they preferentially bind high-mannose-type glycans. Recently, the recombinant Fbs1 derivative protein has been developed as a tool for comprehensive enrichment ofN-glycopeptides. The labeled Fbs3 is also available as a tool for detecting organelle damage in cells as it has characteristic properties which cause it to quickly accumulate in damaged organelles. In this chapter, we introduce two applications of use for Fbs proteins: the unbiasedN-glycopeptide capture method and the detection of damaged organelles in living cells.