Assignment of zebrafish genetic linkage groups to chromosomes
Assignment of zebrafish genetic linkage groups to chromosomes
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DOI：
10.1159/000093332
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Postlethwait, J. H.
中科院分区：
文献类型：
--
作者：
Phillips, R. B.;Amores, A.;Postlethwait, J. H.
在本报告中，利用荧光原位杂交（FISH）和BAC探针将斑马鱼的遗传连锁组分配到特定的染色体上，这些探针含有映射到每个连锁组（LG）的基因。利用相对大小和臂比的组合来鉴定染色体。最大的遗传图谱通常与最大的染色体相对应，但遗传重组倾向于在较小的染色体和端粒附近升高。含有端粒附近基因的大插入克隆经常与多对染色体的端粒杂交，表明端粒附近存在共享的亚端粒重复dna。来自medaka，斑马鱼，河豚和人类的比较基因定位证据表明，这些物种的连锁群具有重复的原染色体的内容。然而，这些重复的连锁群与相似大小或形态的染色体不相关。这表明在硬骨鱼基因组复制之后发生了相当大的染色体重组。版权所有(c) 2006 S. Karger AG，巴塞尔。
In this report the zebrafish genetic linkage groups are assigned to specific chromosomes using fluorescence in situ hybridization (FISH) with BAC probes containing genes mapped to each linkage group (LG). Chromosomes were identified using a combination of relative size and arm ratios. The largest genetic maps generally corresponded to the largest chromosomes, but genetic recombination tended to be elevated in the smaller chromosomes and near telomeres. Large insert clones containing genes near telomeres often hybridized to telomeres of multiple chromosome pairs, suggesting the presence of shared subtelomeric repetitive DNAs near telomeres. Evidence from comparative gene mapping in medaka, zebrafish, pufferfish, and humans suggests that the linkage groups of these species have the content of duplicate proto-chromosomes. However, these duplicate linkage groups are not associated with chromosomes of similar size or morphology. This suggests that considerable chromosome restructuring occurred subsequent to the genome duplication in teleosts. Copyright (c) 2006 S. Karger AG, Basel.