Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ
Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ
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DOI：
10.1007/978-1-4939-3786-8_9
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Tsuji, Takashi
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文献类型：
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作者：
Tezuka, Katsunari;Toyoshima, Koh-ei;Tsuji, Takashi
毛囊形态发生首先由发育中的胚胎中的上皮-间充质相互作用诱导。在毛囊中，各种干细胞群体维持在专门的小生境中以促进重复的毛囊形态发生，这在毛囊的可变下部区域中观察到作为出生后毛发周期。相反，大多数器官的发生在胚胎发生过程中仅被诱导一次。我们开发了一种新的生物工程技术，器官胚芽法，采用三维干细胞培养再生各种器官和再现胚胎器官发生。在这一章中，我们描述了一个协议的毛囊生殖重建成人毛囊来源的上皮干细胞和毛乳头细胞与皮内移植的生物工程毛囊器官胚芽。该方案不仅可用于毛发再生的临床研究，而且可用于干细胞生物学和器官发生的研究。
Hair follicle morphogenesis is first induced by epithelial-mesenchymal interactions in the developing embryo. In the hair follicle, various stem-cell populations are maintained in specialized niches to promote repetitive hair follicle-morphogenesis, which is observed in the variable lower region of the hair follicle as a postnatal hair cycle. In contrast, the genesis of most organs is induced only once during embryogenesis. We developed a novel bioengineering technique, the Organ Germ Method, that employs three-dimensional stem cell culture for regenerating various organs and reproducing embryonic organogenesis. In this chapter, we describe a protocol for hair follicle germ reconstitution using adult follicle-derived epithelial stem cells and dermal papilla cells with intracutaneous transplantation of the bioengineered hair-follicle organ germ. This protocol can be useful not only for the clinical study of hair regeneration but also for studies of stem cell biology and organogenesis.