A procedure for direct lumbar puncture in rats
A procedure for direct lumbar puncture in rats
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DOI：
10.1016/s0361-9230(02)00866-3
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发表时间：
2002-11-30
影响因子：
3.8
通讯作者：
Paíno, CL
中科院分区：
文献类型：
--
作者：
De la Calle, JL;Paíno, CL
我们已经着手建立一种快速，简单和无害的方法，用于重复获得脑脊液（CSF）样品，从正在经历不同的实验程序或患有病理条件的大鼠。在这里，我们报告了一种方法，该方法已被优化为通过直接腰椎穿刺重复收集大鼠30-50穆尔CSF，该方法通常需要15-20分钟才能完成，对动物的危害很小。将大鼠用异氟烷麻醉，并以脊柱在L3-L5椎骨水平弯曲的方式放置在板上。在背部皮肤上进行小切口后，将新生儿腰椎穿刺针引入鞘内空间，并将CSF被动收集在针杯中（本文描述了促进操作）。此外，我们进一步调整了这种方法，以允许鞘内递送药理学试剂和细胞悬液。在这样的实验中，行为测试可以在注射后10-15分钟进行。在鞘内注射后，植入细胞的活性可以通过在稍后的时间对腰椎CSF取样来评估。(C)2002年爱思唯尔科技有限公司All rights reserved.
We have set out to establish a fast, simple and innocuous method for repeatedly obtaining cerebrospinal fluid (CSF) samples from rats that are undergoing different experimental procedures or suffering pathological conditions. Here, we report a method that has been optimized to repeatedly collect 30-50 mul of CSF in rats by direct lumbar puncture using a procedure that generally fakes 15-20 min to perform and presents very little hazard to the animal. The rats are anaestheiized with isofluorane and placed on a board in such a way that the spine is curved at the level of the L3-L5 vertebrae. After performing a small incision in the skin of the back, a neonatal lumbar puncture needle is introduced into the intrathecal space and CSF is passively collected in the needle cup (facilitating maneuvers are described herein). Moreover, we have further adapted this method to permit the intrathecal delivery of pharmacological agents and cell suspensions. In such experiments, behavioral tests can be conducted 10-15 min after the. intrathecal injection and the activity of the implanted cells can be assessed by sampling lumbar CSF at later times. (C) 2002 Elsevier Science Inc. All rights reserved.