Biodetoxification of aflatoxin B1 in cottonseed meal by fermentation of Cellulosimicrobium funkei in duckling diet
Biodetoxification of aflatoxin B1 in cottonseed meal by fermentation of Cellulosimicrobium funkei in duckling diet
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鸭饲料中纤维素微菌发酵对棉籽粕中黄曲霉毒素B1的生物脱毒
DOI：
10.3382/ps/pew352
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发表时间：
2017-04-01
期刊：
影响因子：
4.4
通讯作者：
Qi, D. -S.
中科院分区：
文献类型：
--
作者：
Liu, J.;Song, W. -j.;Qi, D. -S.
进行了两项实验，以优化芬克纤维微生物（C. funkei）对棉籽粕的发酵，以了解该细菌降解黄曲霉毒素B1（AFB1）的能力，然后评估细菌对小鸭的解毒作用。实验1中，通过改变接种量10%（10(8) cfu/mL），料水比1:0.5，温度35℃，反应时间144 h，改善了C. funkei对棉籽粕的发酵，AFB1的生物降解率为83.4%。实验二将112只1日龄雄性樱桃谷小鸭随机分为4个实验组，每组4个重复，每组7只。在两周的时间里，对照组接受基础日粮（BD），第二组接受受10％AFB1棉籽粕污染的基础日粮（96.8μg AFB1/kg），第三组饲喂添加有5％未发酵和5％发酵AFB1污染的棉籽粕的基础日粮（57.0μg AFB1/kg），第四组饲喂添加有10％AFB1棉籽粕的基础日粮（57.0μg AFB1/kg）。 AFB1污染的发酵棉籽粕（16.0μg AFB1/kg）。分析生长性能、相对器官重量和血清生化。结果表明，第1周和第2周，第二组饲料转化率低于对照组（P < 0.05）。此外，2周后，第2组鸭的肝脏、肾脏和脾脏相对重量增加，丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性增加，血尿素氮（BUN）和肌酐（Crt）浓度增加，法氏囊相对重量降低（P < 0.05）。此外，血清中总蛋白（TP）和白蛋白（ALB）浓度在第1周和第2周也显着升高（P < 0.05）。当日粮中 5% 或 10% 的发酵棉籽粕替代等量的未发酵棉籽粕时，这些变化就会减弱或预防。总之，C. funkei 对 AFB1 污染的饲料原料进行发酵为减少黄曲霉中毒对雏鸭的负面影响提供了一种新策略。
Two experiments were conducted to optimize the fermentation of cottonseed meal by Cellulosimicrobium funkei (C. funkei) for the ability of the bacteria to degrade aflatoxin B1 (AFB1) and then to evaluate the bacterial detoxification in ducklings. In experiment 1, the fermentation of cottonseed meal by C. funkei was improved by changing the inoculation amounts by 10% (10(8) cfu/mL), using a 1: 0.5 material to water ratio at 35 degrees C temperature for a 144 h reaction duration, which resulted in an 83.4% biodegradation of AFB1. In experiment 2, 112 one-day-old male Cherry Valley ducklings were randomly allocated to 4 experimental groups with 4 replicates of 7 birds each. For a period of 2 wk the controls received a base duckling diet (BD), a second group received a base diet contaminated with 10% AFB1 cottonseed meal (96.8 mu g AFB1/kg), a third group was fed a base diet added with 5% unfermented and 5% fermented AFB1-contaminated cottonseed meal (57.0 mu g AFB1/kg), and the fourth group was fed a base diet added with 10% AFB1-contaminated fermented cottonseed meal (16.0 mu g AFB1/kg). The growth performance, relative organ weights, and serum biochemistry were analyzed. The results showed that the feed conversion ratio in the second group was lower than that of the controls at wk one and 2 (P < 0.05). Also, after 2 wk, group 2 ducklings had increased relative weights of the liver, kidneys, and spleen, increased activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), increased concentration of blood urea nitrogen (BUN) and creatinine (Crt), and decreased relative weight of Fabricius bursa (P < 0.05). In addition, the concentrations of total protein (TP) and albumin (ALB) in serum were also significantly higher at weeks one and 2 (P < 0.05). These alterations were attenuated or prevented when 5 or 10% fermented cottonseed meal substituted equal amounts of unfermented cottonseed meal in the diet. In conclusion, fermentation of AFB1-contaminated feed materials by C. funkei offers a new strategy to reduce the negative effects of aflatoxicosis in ducklings.