Induction and expression of amphiregulin in human pancreatic cancer.
Induction and expression of amphiregulin in human pancreatic cancer.
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发表时间：
1994-08
期刊：
影响因子：
11.2
通讯作者：
Matthias P. A. Ebert;M. Yokoyama;M. Kobrin;H. Friess;Martha E. Lopez;M. Büchler;G. Johnson;M. Korc
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作者：
Matthias P. A. Ebert;M. Yokoyama;M. Kobrin;H. Friess;Martha E. Lopez;M. Büchler;G. Johnson;M. Korc
表皮生长因子受体由包括生长因子双调蛋白（AR）的多肽家族激活。使用北方印迹分析和聚合酶链反应，我们现在报告，AR mRNA在人胰腺癌细胞系中表达，并且这种表达在其中几个细胞系中被十四酰佛波醇乙酸酯和转化生长因子α增强。AR在正常胰腺组织和恶性胰腺组织中均有表达。然而，在正常胰腺，AR免疫染色是最明显的导管细胞核。相反，在许多癌中，AR也存在于导管样癌细胞的细胞质中。AR的细胞质定位与更晚期的临床分期相关。这些发现表明，AR可能有助于人类胰腺癌中表皮生长因子受体的异常激活，并可能促进疾病进展。
The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregullin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor alpha. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.