Genome maps of simian virus 40 defectives propagated in human glioblastoma cells.
Genome maps of simian virus 40 defectives propagated in human glioblastoma cells.
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在人胶质母细胞瘤细胞中传播的猿猴病毒 40 缺陷型的基因组图谱。
DOI：
10.1016/0042-6822(78)90164-2
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发表时间：
1978
期刊：
影响因子：
3.7
通讯作者：
F. O'Neill
中科院分区：
文献类型：
--
作者：
D. Carroll;F. O'Neill
通过限制酶消化分析以及与野生型基因组的杂交，对在人胶质母细胞瘤细胞 (A172) 上繁殖的四个有缺陷的 SV40 基因组中存在的 DNA 序列进行了表征。与在猴细胞上生长的缺陷DNA一样，这些分子比野生型DNA短10%至20%，并且包含无缺陷基因组特定片段的重复，包括病毒复制起点的多个拷贝。然而，与猴细胞缺陷型不同，在A172细胞上生长的那些也保留了图谱位置0.15处的BamHI位点周围的病毒DNA区域的多个拷贝。这些缺陷仅由源自 SV40 的序列组成，并且不包含可检测到的宿主细胞序列。
The DNA sequences present in four defective SV40 genomes propagated on human glioblastoma cells (A172) have been characterized by analysis of restriction enzyme digests and by hybridization to the wild-type genome. Like defectives grown on monkey cells, these molecules are 10 to 20% shorter than wild-type DNA and contain reiterations of specific segments of the nondefective genome, including multiple copies of the viral replication origin. However, unlike the monkey cell defectives, those grown on A172 cells have also retained multiple copies of the region of viral DNA around theBamHl site at map position 0.15. These defectives consist only of sequences derived from SV40 and contain no detectable host cell sequences.