Induction of follicle formation in long-term cultured normal human thyroid cells treated with thyrotropin stimulates iodide uptake but not sodium/iodide symporter messenger RNA and protein expression
Induction of follicle formation in long-term cultured normal human thyroid cells treated with thyrotropin stimulates iodide uptake but not sodium/iodide symporter messenger RNA and protein expression
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DOI：
10.1677/joe.0.1670125
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发表时间：
2000-10-01
影响因子：
4
通讯作者：
Hershman, JM
中科院分区：
文献类型：
--
作者：
Kogai, T;Curcio, F;Hershman, JM
甲状腺细胞内钠/碘同调体（NIS）对碘的摄取对甲状腺激素的产生至关重要。据报道，在甲状腺疾病，包括甲状腺癌和先天性甲状腺功能减退症中，NIS活性降低。由于缺乏合适的体外模型，对甲状腺细胞碘摄取的研究一直受到限制。最近开发了一种新的培养技术，可以使正常的人甲状腺原代培养细胞生长为单层细胞并表达分化功能超过3个月。我们用这种技术研究了卵泡形成和TSH对这些细胞碘吸收的影响。单层生长的细胞对碘的吸收量很低。卵泡形成是由单层细胞诱导的，电子显微镜显示了卵泡中的细胞极性。单细胞经TSH处理或不经TSH诱导卵泡形成后，碘摄取未见显著增加。然而，卵泡TSH刺激显著增加碘的摄取(相似的4.4倍；P
Iodide uptake by the sodium/iodide symporter (NIS) in thyrocytes is essential for thyroid hormone production. Reduced NIS activity has been reported in thyroid diseases, including thyroid cancer and congenital hypothyroidism. The study of iodide uptake in thyrocytes has been limited by the availability of appropriate in vitro models, A new culture technique was recently developed that allows normal human thyroid primary culture cells to grow as monolayer cells and express differentiated functions for more than 3 months. We used this technique to study the effect of follicle formation and TSH on iodide uptake in these cells. Iodide uptake by the cells grown in monolayer was very low. Follicle formation was induced from monolayer cells, and electron micrographs demonstrated cell polarity in the follicles. No significant increase in iodide uptake was observed after TSH treatment of cells in monolayer or when follicle formation was induced without TSH. TSH stimulation of follicles, however, significantly increased iodide uptake (similar to4.4-fold; P