The blood of Ascidia nigra: blood cell frequency distribution, morphology, and the distribution and valence of vanadium in living blood cells.
The blood of Ascidia nigra: blood cell frequency distribution, morphology, and the distribution and valence of vanadium in living blood cells.
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海鞘血液：血细胞频率分布、形态以及活血细胞中钒的分布和价态。
DOI：
10.2307/1540683
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
W. A. Curby
中科院分区：
文献类型：
--
作者：
K. Kustin;D. Levine;G. C. Mcleod;W. A. Curby
1.对黑囊海鞘的血浆进行了pH、盐度和紫外-可见吸收特性的研究。对黑曲霉全血进行了细胞计数、细胞体积、血红蛋白(铁)含量、钒含量的测定。对血细胞类型进行了检查、分类和差异计数。EPR和核磁共振研究证明了三价钒的存在。用OsO4试剂对全血玻片进行染色，确定载钒细胞主要为绿色球状血细胞。观察到的UV-Vis吸收光谱与已知的钒(III)络合物光谱没有很好的相关性。已经实现了血细胞的密度分离；这一结果结合不同的细胞形态表明，不同的细胞可能代表发育过程中不同的成熟阶段。对不同密度的细胞层进行的钒分析表明，钒可能存在于一种以上类型的血细胞中，而当不以范氏形式存在时，钒可能存在于多种血细胞中。
1. The blood plasma of Ascidia nigra has been characterized with regard to pH, salinity, and ultraviolet-visible absorption. Whole blood of A. nigra has been characterized with regard to cell count, cellular volume, hemoglobin (iron) content, vanadium content. Blood cell types have been examined, categorized, and differentially counted.2. Epr and nmr studies prove that trivalent vanadium is present. Staining of whole blood slides with OsO4 reagent identify the vanadium-carrying cells as being mainly the green globular blood cells. The observed uv-vis absorption spectrum does not correlate well with known vanadium(III) complex spectra.3. Density separation of blood cells has been achieved; this result coupled with various cell morphologies suggests that different cells may represent different maturational stages in a developmental process.4. Vanadium analyses of layerings of cells of different densities suggest that vanadium may be present in more than one type of blood cell, where, when not present as van...