The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis
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DOI：
10.1186/s12885-018-4064-8
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发表时间：
2018-02-07
期刊：
影响因子：
3.8
通讯作者：
Kim, Seong-Hyop
中科院分区：
文献类型：
--
作者：
Lim, Jeong-Ae;Oh, Chung-Sik;Kim, Seong-Hyop
背景：为了阐明麻醉药物对肿瘤免疫的影响，我们评估了异丙酚和七氟醚对乳腺癌患者自然杀伤细胞（NK）、细胞毒性T淋巴细胞（CTL）计数和凋亡率的影响，以及乳腺癌手术患者免疫细胞共培养的影响。方法：对乳腺癌手术患者在诱导麻醉后及术后1、24 h采集静脉血。患者被随机分配到以异丙酚或七氟醚为基础的麻醉组。通过与乳腺癌细胞系共培养，对两组乳腺癌患者的癌细胞、NK细胞和CTL细胞凋亡进行计数和检测。我们还评估了围手术期细胞因子肿瘤坏死因子- α、白细胞介素(IL)-6和IL-10的变化。结果：44例患者纳入最终分析。两组间NK细胞计数、CTL计数及凋亡率均无差异。此外，乳腺癌细胞共培养中发生凋亡的乳腺癌细胞数量在两组之间没有差异。各组间细胞因子未见变化。结论：尽管基础科学研究表明异丙酚在癌症手术中比挥发性药物有潜在的益处，但在临床环境中，在乳腺癌手术麻醉期间，包括乳腺癌细胞在内的NK和CTL细胞计数和凋亡率方面，异丙酚并不优于七氟醚。
Background: To clarify the effect of anaesthetic agents on cancer immunity, we evaluated the effects of propofol and sevoflurane on natural killer (NK) cell, cytotoxic T lymphocyte (CTL) counts and apoptosis rate in breast cancer and immune cells co-cultures from patients who underwent breast cancer surgery.Methods: Venous blood samples were collected after inducing anaesthesia and at 1 and 24 h postoperatively in patients who had undergone breast cancer surgery. The patients were allocated randomly to the propofol-or sevoflurane-based anaesthesia groups. We counted and detected apoptosis in cancer cell, NK cell and CTL of patients with breast cancer by co-culture with a breast cancer cell line in both groups. We also evaluated changes in the cytokines tumour necrosis factor-alpha, interleukin (IL)-6 and IL-10 during the perioperative period.Results: Forty-four patients were included in the final analysis. No difference in NK cell count, CTL count or apoptosis rate was detected between the groups. Furthermore, the number of breast cancer cells undergoing apoptosis in the breast cancer cell co-cultures was not different between the groups. No changes in cytokines were detected between the groups.Conclusion: Although basic science studies have suggested the potential benefits of propofol over a volatile agent during cancer surgery, propofol was not superior to sevoflurane, on the aspects of NK and CTL cells counts with apoptosis rate including breast cancer cell, during anaesthesia for breast cancer surgery in a clinical environment.