Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system.
Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system.
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DOI：
10.1016/j.chroma.2007.01.009
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发表时间：
2007-06
期刊：
影响因子：
--
通讯作者：
Yang Zhao;Q. Du
中科院分区：
文献类型：
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作者：
Yang Zhao;Q. Du
向日葵油-乙醇非水溶剂体系适合于低极性组分的分离。在此溶剂体系下，用1100 mL内径为5.7mm的低速旋转逆流色谱柱分离了3g含15%茄尼醇的烟叶提取物。螺旋形PTFE管。分离得到1.5g黄色油状产物，其中茄尼醇含量为26.8%。烟叶提取液经慢速旋转逆流色谱分离后，除去了焦油等主要杂质。该分离工艺可发展为生产食品级茄尼醇产品的技术。
Non-aqueous solvent system composed of sunflower oil–ethanol was found to be suitable for the separation of lower polarity components. Employing this solvent system, 3g of tobacco leaves extract containing 15% solanesol was separated using a slow rotary counter-current chromatograph equipped with an 1100mL column made of 5.7mm I.D. convoluted PTFE tube. The separation yielded 1.5g of yellow oily product in which solanesol occupied 26.8% determined by HPLC. The tobacco tar and other main impurities in the tobacco leaves extract were removed after slow rotary counter-current chromatographic separation. The separation processing may be developed as a technique for producing a solanesol product with food grade.