Effects of ablation of the pars intercerebralis on ecdysteroid quantities and yolk protein expression in the blow fly, Protophormia terraenovae.
Effects of ablation of the pars intercerebralis on ecdysteroid quantities and yolk protein expression in the blow fly, Protophormia terraenovae.
复制标题
脑间部消融对绿头蝇 Protophormia terraenovae 脱皮类固醇数量和卵黄蛋白表达的影响。
DOI：
10.1111/phen.12012
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发表时间：
2013
影响因子：
1.5
通讯作者：
Ayako Tanaka et al.
中科院分区：
文献类型：
--
作者：
Umezawa;T.;Seiji Akimoto，Toshiyuki Shinoda，Min Chen，Suleyman I. Allakverdiev，Tatsuya Tomo;水波誠;Ayako Tanaka et al.
比较了在生殖（LD 18：6 h，25 °C）和滞育（LD 12：12 h，20 °C）条件下，丽蝇Protophormia terraenovaeRobineau-Desvoidy（双翅目：丽蝇科）血淋巴和卵巢中蜕皮激素的释放量。消融的pars intercerebralis或卵巢上蜕皮激素的数量和消融的pars intercerebralis上蛋黄蛋白表达的影响进行了检查。在生殖条件下，卵黄发生期雌虫的蜕皮激素水平较高，而卵黄发生前雌虫和卵巢成熟的雌虫的蜕皮激素水平较低。在滞育条件下，血淋巴和卵巢中都有少量蜕皮激素。血淋巴中的蜕皮类固醇滴度不受卵巢去除的显著影响，表明卵巢以外的组织也参与蜕皮类固醇的产生。实验性消融大脑间脑部的生殖雌性动物的蜕皮激素水平与假手术或完整雌性动物无显著差异。然而，蛋黄蛋白在脂肪体中的表达被抑制后，去除大脑间部。这些结果表明，蜕皮激素水平的抑制在血淋巴和卵巢与生殖滞育，和大脑间部可以发挥作用，在卵黄蛋白合成不介导蜕皮激素的生产。
Quantities of ecdysteroid are compared in the haemolymph and ovaries of the blowflyProtophormia terraenovaeRobineau‐Desvoidy (Diptera: Calliphoridae) under reproductive (LD 18 : 6 h at 25 °C) and diapause (LD 12 : 12 h at 20 °C) conditions. The effects of ablation of the pars intercerebralis or ovaries on ecdysteroid quantities and of ablation of the pars intercerebralis on yolk protein expression are examined. Under reproductive conditions, the levels of ecdysteroid in vitellogenic females are high, although the levels in previtellogenic females and females with mature ovaries are low. Under diapause conditions, there are low quantities of ecdysteroid in both the haemolymph and ovaries. Ecdysteroid titres in the haemolymph are not significantly affected by the removal of the ovaries, suggesting that tissues other than the ovaries are also involved in the production of ecdysteroids. Reproductive females in which the pars intercerebralis of the brain is experimentally ablated have ecdysteroid levels that are not significantly different from sham‐operated or intact females. However, yolk protein expression in the fat body is suppressed after removal of the pars intercerebralis. These results suggest that the suppression of ecdysteroid levels in the haemolymph and ovaries is associated with reproductive diapause, and that the pars intercerebralis could play a role in yolk protein synthesis without mediating ecdysteroid production.