Isolation and characterization of the Drosophila translational elongation factor 2 gene.
Isolation and characterization of the Drosophila translational elongation factor 2 gene.
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果蝇翻译延伸因子 2 基因的分离和表征。
DOI：
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发表时间：
1989
影响因子：
14.9
通讯作者：
F. C. Kafatos
中科院分区：
文献类型：
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作者：
Y. Grinblat;Nicholas H. Brown;F. C. Kafatos
我们已经分离出一个编码果蝇伸长因子2 （EF2）的cDNA克隆，这是一种参与蛋白质合成的伸长步骤的蛋白质。该鉴定是基于其氨基酸序列与仓鼠EF2的高度同源性（大于80%）。编码果蝇EF2的基因位于2L染色体臂的39E-F位置，可能与M(2)H位点相同，该位点突变时产生Minute表型。基因座的基因组组织包括四个外显子。与多种GTP结合蛋白共享的保守序列片段位于蛋白质的氨基末端的三分之一，而EF2及其原核功能同源物EF-G所特有的片段位于羧基末端的一半；这两个区域在两个各自的外显子中分离。
We have isolated a cDNA clone that encodes the Drosophila melanogaster elongation factor 2 (EF2), a protein involved in the elongation step of protein synthesis. This identification was based on the high degree of its amino acid sequence identity (greater than 80%) to that of hamster EF2. The gene encoding Drosophila EF2 is found at position 39E-F of the 2L chromosomal arm and maybe identical to the M(2)H locus, which produces a Minute phenotype when mutated. The genomic organization of the locus includes four exons. Conserved sequence segments shared with a variety of GTP binding proteins are found in the amino terminal third of the protein, and segments unique to EF2 and its prokaryotic functional homolog, EF-G, are in the carboxy terminal half; these two regions are segregated in two respective exons.