Telomeric repeat-containing RNA structure in living cells
Telomeric repeat-containing RNA structure in living cells
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DOI：
10.1073/pnas.1001177107
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发表时间：
2010-08-17
影响因子：
11.1
通讯作者：
Komiyama, Makoto
中科院分区：
文献类型：
--
作者：
Xu, Yan;Suzuki, Yuta;Komiyama, Makoto
最近在哺乳动物细胞中发现了含有端粒重复序列的RNA（简称TERRA），一种非编码RNA分子。人类染色体末端的 TERRA RNA 的详细结构特征和功能仍不清楚，尽管这种 RNA 分子可能是端粒机制的关键组成部分。在本研究中，我们研究了活细胞中人类 TERRA RNA 的结构特征。使用光开关芘探针，我们发现人TERRA RNA在活细胞中形成平行的G-四联体结构，为人TERRA RNA中存在G-四联体提供了体内证据。此外，成像实验清楚地表明 TERRA RNA G-四链体定位于细胞核的端粒 DNA。这些结果为了解人类 TERRA RNA 的结构和功能提供了有价值的信息。
Telomeric repeat-containing RNA (referred to as TERRA), a non-coding RNA molecule, has recently been found in mammalian cells. The detailed structural features and function of the TERRA RNA at human chromosome ends remain unclear, although this RNA molecule may be a key component of the telomere machinery. In the present studies, we investigated the structural features of human TERRA RNA in living cells. Using a light-switching pyrene probe, we found that human TERRA RNA forms a parallel G-quadruplex structure in living cells, providing the in vivo evidence for the presence of the G-quadruplex in human TERRA RNA. Furthermore, imaging experiments clearly show that TERRA RNA G-quadruplex localizes to telomere DNA at cell nuclei. These results provide valuable information to allow understanding of the structure and function of human TERRA RNA.