Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope
Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope
复制标题
光纤内窥镜对人肺内革兰氏阳性菌的分子检测
DOI：
10.1007/s00259-020-05021-4
复制
发表时间：
2020-09-11
影响因子：
9.1
通讯作者：
Stone, James M.
中科院分区：
文献类型：
--
作者：
Mills, Bethany;Megia-Fernandez, Alicia;Stone, James M.
Purpose The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, a better understanding of bacterial colonization and infection. To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber-based delivery/imaging endoscopic device. Methods We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system. Results The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging ofS. aureuswithin the alveolar space of whole ex vivo human lungs within 60 s of delivery into the field-of-view, using the novel imaging/delivery endomicroscopy device. Conclusion This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.
