Mitotic cyclin Clb4 is required for the intracellular adaptation to glucose starvation in Saccharomyces cerevisiae.
Mitotic cyclin Clb4 is required for the intracellular adaptation to glucose starvation in Saccharomyces cerevisiae.
复制标题
有丝分裂细胞周期蛋白 Clb4 是酿酒酵母细胞内适应葡萄糖饥饿所必需的。
DOI：
10.1002/1873-3468.13722
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Karita S.
中科院分区：
文献类型：
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作者：
Umekawa M;Shiraishi D;Fuwa M;Sawaguchi K;Mashima Y;Katayama T;Karita S.
响应于葡萄糖可用性的细胞内稳态通过各种生理过程（包括细胞增殖、转录和代谢）的紧密协调来维持。在这项研究中，我们使用芽殖酵母Saccharomyces golae来鉴定与碳源依赖性生理过程调节有关的蛋白质。我们发现，有丝分裂细胞周期蛋白Clb4是通过雷帕霉素复合物1的靶点对葡萄糖饥饿反应途径进行最佳调节所必需的。缺乏Clb4的细胞的特征在于自噬失调和细胞大小调节受损。值得注意的是，在长时间的葡萄糖饥饿后，Clb4的破坏严重降低了细胞活力。我们的结论是，Clb 4除了在细胞周期中发挥作用外，还在细胞内对葡萄糖饥饿的适应中发挥作用。
Cellular homeostasis in response to glucose availability is maintained through the tight coordination of various physiological processes, including cell proliferation, transcription, and metabolism. In this study, we use the budding yeastSaccharomyces cerevisiaeto identify proteins implicated in carbon source‐dependent modulation of physiological processes. We find that the mitotic cyclin Clb4 is required for optimal regulation of glucose‐starvation‐responsive pathways through the target of rapamycin complex 1. Cells lacking Clb4 are characterized by dysregulation of autophagy and impaired modulation of cell size. Notably, cell viability after prolonged glucose starvation is severely reduced by disruption of Clb4. We conclude that Clb4, in addition to its function in the cell cycle, plays a role in the intracellular adaptation to glucose starvation.