Monolithic porous polymer stationary phases in polyimide chips for the fast high-performance liquid chromatography separation of proteins and peptides
Monolithic porous polymer stationary phases in polyimide chips for the fast high-performance liquid chromatography separation of proteins and peptides
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DOI：
10.1016/j.chroma.2008.03.025
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发表时间：
2008-07-18
影响因子：
4.1
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
--
作者：
Levkin, Pavel A.;Eeltink, Sebastiaan;Frechet, Jean M. J.
聚（甲基丙烯酸月桂酯-共-乙烯二甲基丙烯酸酯）和聚（苯乙烯-共-二乙烯基苯）固定相的整体格式已制备由热引发的自由基聚合聚酰亚胺芯片内的通道具有的横截面为200 μ m × 200 μ m和长度为6.8厘米。然后，这些芯片用于分离蛋白质的混合物，包括核糖核酸酶A，肌红蛋白，细胞色素c和卵清蛋白，以及肽。通过UV吸附监测分离。基于甲基丙烯酸酯和苯乙烯化学的整体相都能够快速基线分离大多数测试混合物。最好的性能是实现与苯乙烯单体快速基线分离的所有四种蛋白质在不到2.5分钟。在原位整料制备过程中提供微流控装置表现出良好的批间和注射注射重复性。(c)2008 Elsevier B. V.保留所有权利。
Poly(lauryl methacrylate-co-ethylene dimethacrylate) and poly(styrene-co-divinylbenzene) stationary phases in monolithic format have been prepared by thermally initiated free radical polymerization within polyimide chips featuring channels having a cross-section of 200 mu m x 200 mu m and a length of 6.8 cm. These chips were then used for the separation of a mixture of proteins including ribonuclease A, myoglobin, cytochrome c, and ovalbumin, as well as peptides. The separations were monitored by UV adsorption. Both the monolithic phases based on methacrylate and on styrene chemistries enabled the rapid baseline separation of most of the test mixtures. Best performance was achieved with the styrenic monolith leading to fast baseline separation of all four proteins in less than 2.5 min. The in situ monolith preparation process affords microfluidic devices exhibiting good batch-to-batch and injection-to-injection repeatability. (c) 2008 Elsevier B.V. All rights reserved.