Nitrite-cured meats as a source of endogenously and exogenously formed N-nitrosoproline in the ferret.
Nitrite-cured meats as a source of endogenously and exogenously formed N-nitrosoproline in the ferret.
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亚硝酸盐腌制的肉是雪貂体内内源和外源形成的 N-亚硝基脯氨酸的来源。
DOI：
10.1016/0278-6915(89)90005-7
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hotchkiss,JH
中科院分区：
文献类型：
--
作者：
Perciballi,M;Conboy,JJ;Hotchkiss,JH
将含有 120 毫克 Na15NO2/kg 的亚硝酸盐腌肉喂给雄性雪貂 (Mustela putorius Furo)。在食用肉期间，给动物口服 0.87 mmol [2-2H]脯氨酸。在整个研究过程中收集所有尿液，并通过质谱法分析总 N-亚硝基脯氨酸 (NPRO) 和 NPRO 的同位素富集。腌肉饮食使 NPRO 的尿排泄量增加了 14 倍。同位素分析表明，大约 70% 的 NPRO 来自腌制肉，其中大部分在分析上不可用或“结合”在肉中。一小部分排出的 NPRO 似乎是由于摄入腌肉而在胃中形成的。少量排出的 NPRO 不包含任何同位素标记的原子。施用抗坏血酸并没有显着改变 NPRO 的排泄。动物口服 11.4 μmol N 末端脯氨酸被亚硝化的肽后，在接下来的 48 小时内 NPRO 的排泄量增加了 385 nmol。这些数据表明，亚硝酸盐腌制的肉含有结合的 NPRO，这构成了尿液中 NPRO 的总量。
Nitrite-cured meat containing 120 mg Na15NO2/kg was fed to male ferrets (Mustela putorius furo). During consumption of the meat, the animals were dosed orally with 0.87 mmol [2-2H]proline. All urine was collected throughout the study and analysed for totalN-nitrosoproline (NPRO) and isotopic enrichment of NPRO by mass spectrometry. The cured-meat diet increased the urinary excretion of NPRO 14-fold. Isotope analyses indicated that approximately 70% of the NPRO came from the cured meat, the majority of which was analytically unavailable or ‘bound’ NPRO in the meat. A small portion of the excreted NPRO appeared to be formed in the stomach as a result of ingesting the cured meat. A minor amount of the excreted NPRO did not contain any isotopically labelled atoms. The administration of ascorbic acid did not significantly alter NPRO excretion. Animals dosed orally with 11.4 μmol of a peptide in which the N-terminal proline was nitrosated increased their excretion of NPRO by 385 nmol over the following 48 hr. These data indicate that nitrite-cured meat contains bound NPRO which contributes to the total amount of NPRO in the urine.