Preservation effects on stable isotope analysis of fish muscle
Preservation effects on stable isotope analysis of fish muscle
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DOI：
10.1577/1548-8659(2002)131
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发表时间：
2002-03-01
影响因子：
1.4
通讯作者：
Winemiller, KO
中科院分区：
文献类型：
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作者：
Arrington, DA;Winemiller, KO
我们评估了盐和福尔马林-乙醇样品保存对鱼肌肉组织碳和氮同位素特征的影响。我们发现组织保存技术对δ (13)C和δ (15)N的影响具有统计学意义；然而，变化幅度很小，方向均匀。同位素变化与以前使用福尔马林保存鹌鹑血液、肌肉和羊血液样本的研究中观察到的变化相似。由于盐保存引起的同位素变化最小(+0.13 /千δ (13)C, +0.72 /千δ (15)N)，我们建议盐作为一种简单、廉价的保存技术，用于偏远地区采集的生物样品。用福尔马林和乙醇保存的标本受保存的影响最小(-1.12 /千δ (13)C, +0.62 /千δ (15)N)，因此当碳源和氮源的差异超过2 /千时，可能适合稳定同位素分析的生态应用。需要进一步的研究来评估福尔马林固定和酒精保存对鱼类组织同位素特征的潜在长期储存影响。
We evaluated the effect of salt and formalin-ethanol sample preservation on carbon and nitrogen isotopic signatures of fish muscle tissue. We found statistically significant effects of the tissue preservation technique on both delta(13)C and delta(15)N; however, the magnitude of change was small and directionally uniform. Isotopic shifts were similar to those observed in previous studies in which formalin was used to preserve samples of quail blood and muscle and sheep blood. Because salt preservation caused minimal isotopic shifts (+0.13parts per thousand delta(13)C, +0.72parts per thousand delta(15)N), we propose salt as an easy, inexpensive preservation technique for biological samples collected in remote field settings. Specimens preserved with formalin and ethanol were minimally affected by preservation (-1.12parts per thousand delta(13)C, +0.62parts per thousand delta(15)N) and therefore may be suitable for ecological applications of stable isotope analysis when carbon and nitrogen sources are differentiated by more than 2parts per thousand. Further research is required to evaluate potential long-term storage effects of formalin fixation and alcohol preservation on isotopic signatures of fish tissues.