Toxicological and analytical investigations of noni (Morinda citrifolia) fruit juice
Toxicological and analytical investigations of noni (Morinda citrifolia) fruit juice
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DOI：
10.1021/jf062130i
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发表时间：
2007-01-24
影响因子：
6.1
通讯作者：
Basar, Simla
中科院分区：
文献类型：
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作者：
Westendorf, Johannes;Effenberger, Katharina;Basar, Simla
众所周知，海巴戟根中含有遗传毒性的蒽醌类化合物。由于诺丽果汁的广泛使用，通过一系列短期测试来检查可能的遗传毒性风险。还对诺丽果汁进行了化学分析，以确定可能存在的蒽醌类化合物。在沙门氏菌微粒体试验中，诺丽汁提取物对TA1537菌株有轻微的诱变作用，这是因为存在类黄酮。用V79中国仓鼠成纤维细胞进行哺乳动物致突变性试验，未观察到致突变性。用诺丽浓缩汁处理的大鼠在原代大鼠肝细胞中没有显示DNA修复合成(UDS)，也没有观察到DNA加合物或DNA链断裂。诺丽果汁中的蒽醌类化合物的高效液相色谱分析为阴性，灵敏度为1ppm。综上所述，化学分析和遗传毒性测试表明，诺丽果汁不具有遗传毒性潜力，而且诺丽果汁中不存在遗传毒性的蒽醌类化合物。
Morinda citrifolia (noni) is known to contain genotoxic anthraquinones in the roots. Because of the widespread use of noni juice, the possible genotoxic risk was examined through a battery of short-term tests. Noni juice was also chemically analyzed for the possible presence of anthraquinones. Noni juice extract in the Salmonella microsome assay showed a slight mutagenic effect in strain TA1537, due to the presence of flavonoids. No mutagenicity was observed in the mammalian mutagenicity test with V79 Chinese hamster fibroblasts. Rats treated with a noni juice concentrate did not show DNA repair synthesis (UDS) in primary rat hepatocytes, nor could DNA adducts or DNA strand breaks be observed. HPLC analysis of noni juice for anthraquinones was negative, with a sensitivity of < 1 ppm. In summary, chemical analysis and genotoxicity tests reveal that noni juice does not have a genotoxic potential and that genotoxic anthraquinones do not exist in noni juice.