Extension of cell life-span and telomere length in animals cloned from senescent somatic cells
Extension of cell life-span and telomere length in animals cloned from senescent somatic cells
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DOI：
10.1126/science.288.5466.665
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发表时间：
2000-04-28
期刊：
影响因子：
56.9
通讯作者：
West, MD
中科院分区：
文献类型：
--
作者：
Lanza, RP;Cibelli, JB;West, MD
克隆的潜力部分取决于该过程是否可以逆转细胞衰老并将体细胞恢复到表型年轻的状态。在这里，我们报告的出生6个健康的克隆小牛来自人口的衰老供体体细胞。核转移延长了衰老细胞的复制寿命（0至4个群体double剩余）大于90个群体double。克隆动物细胞中的早期群体倍增水平互补DNA-1（EPC-1，一种年龄依赖性基因）表达是年龄匹配（5至10个月大）对照细胞的3.5至5倍。Southern杂交和流式细胞术分析表明，端粒也延伸到新生儿以外（
The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative lifespan of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (